We narrowed to 16,889 results for: Por

1. pDONR221-eGFP_mito

   Plasmid

   #221414

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   eGFP_mito
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGK ARF6(Q67L)-CFP

   Plasmid

   #200428

   Purpose

   Mammalian expression of Q67L mutation in ARF6

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   PGK

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB.GFP-KIF1A tail 395-1698

   Plasmid

   #134618

   Purpose

   Mammalian expression of mouse KIF1A 395-1698

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF1A (Kif1a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 394 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-UBC-hCD46-T2A-GFP

   Plasmid

   #205457

   Purpose

   lentiviral plasmid for expression of human CD46

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD46 (CD46 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

   Plasmid

   #90015

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-GFP
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Rab8a-TN

   Plasmid

   #86077

   Purpose

   A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed threonine 22 to aspargine

   Promoter

   CMV

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiCRISPRv1_SDHB

   Plasmid

   #177981

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNLF1-C_hCOL4A5-Nluc_R1683X

   Plasmid

   #198124

   Purpose

   Express hCOL4A5_R1683X in mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Tags

   Nluc

   Expression

   Mammalian

   Mutation

   R1683X

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TetO-FUW-PLZF

   Plasmid

   #61543

   Purpose

   Expresses PLZF in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   PLZF (ZBTB16 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   I380T and G580D

   Promoter

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-RYK-V5/HIS

    Plasmid

    #205610

    Purpose

    Expression of human RYK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RYK receptor tyrosine kinase (RYK Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBa.GFP-KIF1C tail 400-1103

    Plasmid

    #134621

    Purpose

    Mammalian expression of human KIF1C 400-1103

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SOX17-3'gRNA

    Plasmid

    #210465

    Purpose

    gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    SOX17 (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

    Plasmid

    #170177

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid

    #67275

    Purpose

    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

    Insert

    puromycin resistant gene
    

    Use

    Tags

    linked to red flouresent protein gene (katushka)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. APEX2-SOPP3-TM

    Plasmid

    #229077

    Purpose

    Expresses APEX2-SOPP3 in the cell surface

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in the cell surface (APEX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-eGFP_KDEL

    Plasmid

    #221411

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    eGFP_KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

    Plasmid

    #134613

    Purpose

    Mammalian expression of rat KIF5C 378-955

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5C (Kif5c Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG876 pDisplay-pFAST

    Plasmid

    #172868

    Purpose

    expresses pFAST at the surface of mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST-PDGFR
    

    Use

    Tags

    IgK leader sequence (N terminal on insert) and My…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSS1 (hLPL-FLAG)

    Plasmid

    #102447

    Purpose

    Expresses FLAG-tagged human LPL in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    Lipoprotein lipase (LPL Human)
    

    Use

    Tags

    6x His and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTol2CG2-acta1:QFGal4-SV40pA

    Plasmid

    #155121

    Purpose

    Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    acta1:QFGal4
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    acta1

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-Y951F-VEGFR2-mCherry

    Plasmid

    #139607

    Purpose

    Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLentiCRISPRv1_SDHA

    Plasmid

    #177980

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-EFS-DIO-GRAB_Ado1.0med

    Plasmid

    #153292

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Use

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

26. FKBP-beta1(617-949)-GFP

    Plasmid

    #100732

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP1B1 (AP1B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGAPTrap TALEN 2

    Plasmid

    #83369

    Purpose

    Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pGAPTrap-TALEN1

    Plasmid

    #83368

    Purpose

    Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

29. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEN_TT 3xFLAG-KPNB1

    Plasmid

    #192301

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNB1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNB1 (KPNB1 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. eSpCas9-EF-5Nanog_2

    Plasmid

    #174871

    Purpose

    CRISPR vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. hSyn-Aion-citrine

    Plasmid

    #192569

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. VPS13C^halo∆1235-1748

    Plasmid

    #187297

    Purpose

    Express VPS13C truncation mutant in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    VPS13C truncation mutant (VPS13C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

    Plasmid

    #170175

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX-UAP56

    Plasmid

    #157661

    Purpose

    Expresses UAP56 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Spliceosome RNA helicase DDX39B (DDX39B Human)
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-43

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Human Myc-XIAP

    Plasmid

    #217627

    Purpose

    Mammalian expression of Myc tagged XIAP

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1-Y1214F-VEGFR2-mCherry

    Plasmid

    #139609

    Purpose

    Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

    Plasmid

    #90010

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C WT
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LENTICRISPR-TKT_sgRNA2

    Plasmid

    #201625

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LENTICRISPR-TKT_sgRNA1

    Plasmid

    #201624

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3-AktAR

    Plasmid

    #61624

    Purpose

    FRET-based biosensor for monitoring Akt activity

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR (AKT1 Human, Budding Yeast, Synthetic)
    

    Use

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PB-Ef1a-RPS27A-UTR-PP7-WPRE

    Plasmid

    #198339

    Purpose

    PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    RPS27A-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-EFS-DIO-GRAB_OTmut

    Plasmid

    #185389

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. p.UTA.2.0 miR-27a-3p

    Plasmid

    #82438

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Use

    Tags

    Expression

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAAV-CaMKII-soCoChR-GFP

    Plasmid

    #107711

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. WDR5-HaloTag

    Plasmid

    #200881

    Purpose

    Mammalian expression of human WDR5 with C-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    WDR5-HaloTag (WDR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. 6K-loop-->R GLUT1-GFP

    Plasmid

    #200098

    Purpose

    human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 225, 229, 230, …

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. 11K-cyto-->R GLUT1-GFP

    Plasmid

    #200096

    Purpose

    human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 6, 7, 225, 229,…

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Mutation

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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