We narrowed to 10,975 results for: NSI;

1. HR-TERT-promotor

   Plasmid

   #71398

   Purpose

   Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Endogenous TERT promotor

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191155

   Purpose

   Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:DNAJB1.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC_C_LAMP1

   Plasmid

   #111669

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1/Hygro(-)-B2-TS5

   Plasmid

   #104354

   Purpose

   Expresses human beta 2 integrin tension sensor

   Depositor

   Timothy Springer, Pontus Nordenfelt

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   Human integrin b2 with tension sensor (ITGB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEMS-SDHB-Halo7Tag

   Plasmid

   #108973

   Purpose

   Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

   Depositor

   Karin Busch

   Article

   Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

   Insert

   succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
   

   Use

   Tags

   Halo7-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-N1-PHD3

   Plasmid

   #21402

   Depositor

   Eric Metzen

   Article

   Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

   Insert

   EGLN3 (EGLN3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pFastBacDual with LFn + Needle

   Plasmid

   #84868

   Purpose

   Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Needle
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold TDP43 M337V

   Plasmid

   #27453

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa and His6

   Expression

   Bacterial

   Mutation

   Methionine 337 mutated to Valine (M337V)

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

9. PAmCherry1-C1-LACT-C2

   Plasmid

   #202682

   Purpose

   Photoactivatable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   PAmCherry1: Bos taurus MFGE8 (274-431) (MFGE8 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. peGFP C1-SPG20 SC domain

    Plasmid

    #218580

    Purpose

    Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-417

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1-PHD1

    Plasmid

    #21400

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    EGLN2 (EGLN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

12. All-in-one split-ADAR2(E488Q, N496F)

    Plasmid

    #170148

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACE-GIRK2-S

    Plasmid

    #172426

    Purpose

    Expression of full-length, human GIRK2 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101298

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 of alpha-Catenin

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2-sgMyc_2

    Plasmid

    #138346

    Purpose

    Express guide RNA 1 for mouse Myc

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    cMyc sgRNA (Myc Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. trLATmEos3.2

    Plasmid

    #203745

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAT (LAT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-CIBN-EGFP-KRasCT

    Plasmid

    #178527

    Purpose

    Expresses CIBN-EGFP-KRasCT in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CIBN-EGFP-KRasCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS416 Gal M337V YFP

    Plasmid

    #27449

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pBoBi-hALDOA

    Plasmid

    #210806

    Purpose

    For stably expression in cells

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUBQ10:AtFLS2

    Plasmid

    #202186

    Purpose

    Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2 (FLS2 Mustard Weed)
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBeast-pAhpC-RBS- LacZ

    Plasmid

    #190060

    Purpose

    LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    lacZ
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBeast-pT7-soxA

    Plasmid

    #190057

    Purpose

    Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    SoxA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FCK-ArchT-GFP

    Plasmid

    #31177

    Purpose

    Lentiviral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PAmCherry-N1-FRB-Tubbyc

    Plasmid

    #202678

    Purpose

    Photoactivatable and rapamycin recruitable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pINDUCER21-puro_TAOK3

    Plasmid

    #209778

    Purpose

    doxycycline-inducible expression of TAOK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK3 (TAOK3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-REPORT (PGK) 5xGRE-NanoLuc

    Plasmid

    #207170

    Purpose

    GAl4 response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

    Inserts

    NanoLuciferase
    d2eGFP
    5x GRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mutation

    Promoter

    Gal4 response element minP and PGK

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAC_C_ARP2

    Plasmid

    #111658

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    ARP2 (ACTR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX6P1-Ubl

    Plasmid

    #78712

    Purpose

    Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

    Insert

    Human Sacsin 2-85
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAC_C_TOM20

    Plasmid

    #111678

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    TOM20 (TOMM20 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

    Plasmid

    #26569

    Depositor

    Diane Lipscombe

    Article

    Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pINDUCER21-puro_TAOK2

    Plasmid

    #172979

    Purpose

    doxycycline-inducible expression of TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK5 HA-GST-Presc-mAkt1 (wt)

    Plasmid

    #48805

    Purpose

    expresses wt Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pMTTH+H1.4ΔCTD

    Plasmid

    #157797

    Purpose

    Expression of human linker histone H1.4 without c-terminal tail

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4ΔCTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

    Plasmid

    #75132

    Purpose

    Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pCA528 SPASTIN , residues 108-200

    Plasmid

    #180628

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX6P1-sr1(SIRPT1)

    Plasmid

    #75297

    Purpose

    Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

    Insert

    Human Sacsin 84-339
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LifeAct-mAvicFP1

    Plasmid

    #129512

    Purpose

    Expresses LifeAct-mAvicFP1 in mammalian cells

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    LifeAct-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 is AvicFP1 F64L + A206K

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CMV Flag PAI-RBP

    Plasmid

    #45452

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pAAV_12xTEAD_luc2

    Plasmid

    #173004

    Purpose

    firefly luciferase reporter vector with 12x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    12xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge