We narrowed to 9,406 results for: Coli
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Plasmid#140625PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHelA12
Plasmid#176493PurposeExpresses the N-terminal His6-tagged SARS-CoV helicase in E coli.DepositorInsertSARS-CoV NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationV570IPromoterT7Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD2 (OTU, aa 132-314)
Plasmid#61410PurposeExpresses human OTUD2 (OTU domain) in E. coli.DepositorInsertOTUD2 (YOD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-131 and aa 315-348.PromoterT7Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2-N
Plasmid#128308PurposeExpress hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP2 N terminal fragment (OB fold) (NABP2 Human)
UseTagsHisExpressionBacterialMutationN-terminal domain (1–138 aa)PromoterT7Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2NhNABP1C
Plasmid#128313PurposeExpress chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tagDepositorUseTagsHisExpressionBacterialMutationchimeric protein hNABP2N+hNABP1C, Y85C (please se…PromoterT7Available SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRD404
Plasmid#163109PurposeExpression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmNeonGreen_H-NSdbd
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
His 3C Kinase dead MKK6 1-334
Plasmid#87882PurposeE. coli expression of MKK6 kinase deadDepositorInsertMKK6 (MAP2K6 Human)
UseTags3C protease site and 6xHisExpressionBacterialMutationK82A, kinase dead mutantPromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-PD-4xDQNAT
Plasmid#128399PurposePeriplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertH. influenzae protein D
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-69
Plasmid#49815Purposeexpression vector for SRAP(13-69) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-69 (SRA1 Human)
UseTagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A-Q482A
Plasmid#128946Purposeexpresses R480A Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-OiRS3GG
Plasmid#98589PurposeExpresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertsgroup I intron
PheS cassette to be selectively replaced by unique asRNA probes
UseTagsExpressionBacterialMutationPromoterpBAD and pLtetOAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 G domain
Plasmid#74598Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389DepositorInserthuman dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationPromoterT7Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-87
Plasmid#49816Purposeexpression plasmid for SRAP(13-87) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-87 (SRA1 Human)
UseTagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-215
Plasmid#49817Purposeexpression vector for SRAP(13-215) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-215 (SRA1 Human)
UseTagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His6-MBP-TEV-Rgs14
Plasmid#236395PurposeExpresses His6-MBP-TEV-Rgs14 in E. coliDepositorInsertRattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
UseTagshexahistidine (H6) tag and maltose-binding protei…ExpressionBacterialMutationPromoterAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 UBL-12
Plasmid#227230PurposeExpresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationaa 535-775PromoterT7-promoterAvailable SinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-H-V5-His
Plasmid#222850PurposeE. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIV-Streptomyces
Plasmid#226250PurposeExpresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Streptomyces)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
hzHDAC10
Plasmid#225542PurposeExpresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.DepositorInsertHistone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationMutated A24E and D94APromoterT7Available SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2C
Plasmid#225070PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2C)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2A
Plasmid#225068PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2A)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2B
Plasmid#225069PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2B)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-57
Plasmid#225074PurposeExpresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanII-57)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-6
Plasmid#225076PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanIII-6)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-23
Plasmid#225067PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-23)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-A-V5-His
Plasmid#222849PurposeE. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-A
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-CD28 CAR hinge (I114 – P152)
Plasmid#224929PurposeExpression GST fusion CD28 CAR hinge (I114-P152) in Escherichia coli.DepositorInsertCD28 CAR hinge (I114 – P152) (TMIGD2 Human)
UseTagsglutathione-S-transferaseExpressionBacterialMutationPromoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-838
Plasmid#217994PurposepLEXSY_I-blecherry3_dom_lacZ Destination VectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterT7 promotorAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_SAT1 (human)
Plasmid#220477Purposeheterologous expression of SSAT1 under endogenous speG E. coli promoterDepositorAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB-SNAP
Plasmid#166146PurposeGateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10 and SNAPExpressionBacterialMutationPromoterAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB
Plasmid#166147PurposeGateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10-Smt3ExpressionBacterialMutationPromoterAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB-SNAP
Plasmid#166148PurposeGateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5(kf)-SNAP
Plasmid#166149PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-DDX5(kf)-SNAP
Plasmid#166152PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
UseTagsHis10 and SNAPExpressionBacterialMutationPromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-HT(C199S)_WPRE
Plasmid#216415PurposeEncodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectorsDepositorInsertoROS-HT(C199S)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP-eNOSpep-EGFP
Plasmid#205306PurposeExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coliDepositorInsertWHaloCaMP-eNOSpep-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-MerTK-PTPN1
Plasmid#197988PurposeExpression of MerTK Kinase Domain (wild-type) in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mini v2-HF24_F4
Plasmid#195718PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#4 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F20
Plasmid#195734PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#20 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F5
Plasmid#195719PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#5 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F12
Plasmid#195726PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#12 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F3
Plasmid#195717PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#3 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F24
Plasmid#195738PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#24 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F7
Plasmid#195721PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#7 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F8
Plasmid#195722PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#8 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F14
Plasmid#195728PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#14 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits