163,606 results

1. T7-VEE-mCherry

   Plasmid

   #193355

   Purpose

   Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEZYmyc-His

   Plasmid

   #18701

   Purpose

   pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-CMV-Galpha-12 ONE-GO

   Plasmid

   #189739

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-12 ONE-GO
   GNA12 (GNA12 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. His-GFP SNAP25

   Plasmid

   #170872

   Purpose

   Mammalian expression of His-GFP SNAP25.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   SNAP 25 (Snap25 Rat)
   

   Use

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xnls-mNeonGreen

   Plasmid

   #98875

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   3xNLS
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-scFv-GCN4-sfGFP-GB1-dWPRE

   Plasmid

   #60907

   Purpose

   The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRBad-Z-CspGFP

   Plasmid

   #40730

   Depositor

   Brian McNaughton

   Article

   Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

   Insert

   Leucine Zipper prime - C super positive Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBad

   Available Since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. mycBioID2-13X Linker-MCS

   Plasmid

   #92308

   Purpose

   To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Humanized A. aeolicus BioID2 with R40G and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-HA-JMJD3

   Plasmid

   #24167

   Purpose

   Mammalian expression of human JMJD3 with HA tag

   Depositor

   Kristian Helin

   Article

   Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAD-KPS12-Pman

    Plasmid

    #133832

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSII426

    Plasmid

    #35470

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast 2 micron vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET11a-Z-NspGFP

    Plasmid

    #40729

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    N super positive GFP - Leucine Zipper
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDawn-sfGFP

    Plasmid

    #107741

    Purpose

    Expresses superfolder GFP under the control of a blue-light transcriptional regulator

    Depositor

    Ingmar Riedel-Kruse

    Article

    Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLambda

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR v2-dCas9

    Plasmid

    #112233

    Purpose

    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

    Depositor

    Thomas Gilmore

    Article

    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

    Inserts

    dCas9
    Porumycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed: Aspartic acid 10 to Glycine, Histidine 8…

    Promoter

    EFS-NS

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAD-sfGFP(Sp)-Pman

    Plasmid

    #133833

    Purpose

    Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(Sp)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-HIF2alpha-pcDNA3

    Plasmid

    #18950

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCalN-ddGFP-A

    Plasmid

    #40290

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    ddGFP-A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMIEG3-JunDN

    Plasmid

    #40350

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    Jun dominant negative (JUN Human)
    

    Use

    Retroviral

    Tags

    HA and IRES eGFP

    Expression

    Mammalian

    Mutation

    first 122 amino acids deleted

    Promoter

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-myc-FLNa WT

    Plasmid

    #8982

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A (FLNA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138128

    Purpose

    Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162377

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pT3TS-spCas9 ABE-Umax

    Plasmid

    #222138

    Purpose

    Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 (cas9 Synthetic)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. p11-LacY-wtx1

    Plasmid

    #69056

    Purpose

    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

    Depositor

    Huimin Zhao

    Article

    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

    Inserts

    ccdB (ccdB )
    lacY (lacY )
    

    Use

    Tags

    A177C

    Expression

    Mutation

    Promoter

    pBAD and pLac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. MP9956:SFG.GD3synthase-2A-GD2synthase

    Plasmid

    #75013

    Purpose

    Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

    Depositor

    Martin Pule

    Article

    Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

    Inserts

    murine GD3 synthase
    murine GD2 synthase
    

    Use

    Retroviral; Momlv retroviral vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGJJ045

    Plasmid

    #183621

    Purpose

    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TMPRSS2

    Plasmid

    #53887

    Purpose

    Expression of TMPRSS2

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Has Service

    Cloning Grade DNA

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pHAGE2-TetOminiCMV-SV40LT

    Plasmid

    #136616

    Purpose

    Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    SV40 large T antigen
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCTCON2-Aga1p

    Plasmid

    #158141

    Purpose

    Expresses Aga1p in yeast

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge