159,332 results

1. [pSK24] pACYCDuet His-c7orf59|HBXIP

   Plasmid

   #136143

   Purpose

   bacterial expression of c7orf59 and HBXIP

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-sgRNA-RFP

   Plasmid

   #166005

   Purpose

   Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

   Depositor

   Martin Buck

   Article

   Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. IL-8 toehold switch sensor

   Plasmid

   #111908

   Purpose

   Toehold switch sensor to detect IL-8 mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   IL8 toehold switch sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCoofy55

   Plasmid

   #122007

   Purpose

   Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His10-SumoStar (Life Sensors) and Multi2: dependi…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. All-in-one lenti-BE4-NG

   Plasmid

   #200448

   Purpose

   Lentivirus vector for base editing

   Depositor

   Benjamin Cravatt

   Article

   Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_TRAF3_WT

   Plasmid

   #81755

   Purpose

   Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TRAF3 (TRAF3 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET47b-T7-enAsCas12f

   Plasmid

   #204642

   Purpose

   Expression of enAsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   T7

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTC391

   Plasmid

   #91220

   Purpose

   protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUS212

   Plasmid

   #84580

   Purpose

   E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.

   Depositor

   Nicholas Coleman

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCI-neo-cOVA

    Plasmid

    #25097

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    cytoplasmic ovalbumin
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid

    #129631

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-REST_sh_seq1_WPRE

    Plasmid

    #127573

    Purpose

    shRNA to knock down RE1-silencing transcription factor (REST)

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    shRNA for knock down of REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFX-mito-EGFP

    Plasmid

    #159096

    Purpose

    Express EGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-mCherry-sesRNA-GFP-WPRE-pA

    Plasmid

    #192065

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Cheta
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Gdf6

    Plasmid

    #163598

    Purpose

    Expresses GDF-6 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf6 (Gdf6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP3664

    Plasmid

    #222350

    Purpose

    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. phNFM

    Plasmid

    #132597

    Purpose

    Expresses human NFM

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFM (NEFM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiTet-P2A-mCherry BlastR

    Plasmid

    #186743

    Purpose

    Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    P2A-mcherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSDMA25

    Plasmid

    #67940

    Purpose

    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains nourseothricin resistance marker (NAT)

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

    Type

    Empty backbone

    Use

    Cryptococcal complementation vector

    Tags

    Expression

    Mutation

    Promoter

    ACT1 for Cryptococcal marker

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid

    #46911

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP-KRAB fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, HA, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. pIRES2-hPAC

    Plasmid

    #129488

    Purpose

    Expresses human TMEM206

    Depositor

    Zhaozhu Qiu

    Article

    Yang et al Science. 2019 Apr 26;364(6438):395-399.

    Insert

    Transmembrane protein 206 (PACC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mEGFP-C1

    Plasmid

    #54759

    Purpose

    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pJBL703

    Plasmid

    #140373

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-2BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PLlacO-1-mfLon

    Plasmid

    #45152

    Depositor

    Michel Maharbiz

    Article

    Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

    Insert

    Lon
    

    Use

    Synthetic Biology

    Tags

    ssRA degradation (LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    PLlacO-1

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid

    #207773

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. SV-ABAactDA

    Plasmid

    #38247

    Depositor

    Jerry Crabtree

    Article

    Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

    Inserts

    VP16/PYL1
    Gal4DBD/ABI*
    

    Use

    Tags

    Flag, Gal4DBD, HA, and VP16

    Expression

    Mammalian

    Mutation

    D143A

    Promoter

    SV40 (IRES) and SV40p

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMApra-Ptet1

    Plasmid

    #218360

    Purpose

    Empty backbone with tetR-PtetA expression system with single tetR operator site

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid

    #84563

    Purpose

    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    

    Use

    Lentiviral

    Tags

    myc tag (9x)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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