We narrowed to 17,071 results for: Por

1. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115220

   Purpose

   Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   Pin3Cas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115210

   Purpose

   Expresses AspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   AspCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a-LaM4-EGFP

   Plasmid

   #182641

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
   

   Tags

   BAP, EGFP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Circular-ic-CVB3-GFP

   Plasmid

   #226260

   Purpose

   Production of in situ circularizable RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmGFP-Sec16S

   Plasmid

   #15775

   Depositor

   Benjamin Glick

   Article

   Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

   Insert

   Sec16S (SEC16B Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Circular-ic-EMCV-GFP

   Plasmid

   #226259

   Purpose

   Production of in situ circularizable RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-rab9 DN

   Plasmid

   #12664

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB9 (Homo sapiens) (RAB9A Human)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   changed serine 21 to asparagine

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav1-mRFP

   Plasmid

   #14434

   Depositor

   Ari Helenius

   Article

   Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

   Insert

   Caveolin-1 (CAV1 Canis familalis)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Expression

    Yeast

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-hARFGAP1-HA

    Plasmid

    #206409

    Purpose

    Mammalian expression of hARFGAP1

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

    Insert

    ARFGAP1 (ARFGAP1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAIP Nup62Fv2GFP

    Plasmid

    #139684

    Purpose

    Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

    Depositor

    Edward Campbell

    Article

    Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

    Insert

    Nup62 (NUP62 Human)
    

    Use

    Lentiviral

    Tags

    2 copies of GFP and Dimerization domain FKBP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid

    #222101

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Bacteroidetes-bGSDM-TEV ORF
    

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    TEV protease site replaces residues E242–G248

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-FNLSNG-P2A-Puro

    Plasmid

    #136900

    Purpose

    Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAGGS-MCS-NLS-3XFLAG-V5

    Plasmid

    #196383

    Purpose

    Empty backbone vector to insert cDNA for strong expression in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Type

    Empty backbone

    Tags

    3xFLAG and V5

    Expression

    Bacterial and Mammalian

    Promoter

    CAGGs

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLR5-CAG-TetR_W43F-3xmNG

    Plasmid

    #174880

    Purpose

    PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    TetR(W43F)-3xmNeonGreen
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCMV-H2B-FT-Medium

    Plasmid

    #157670

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Medium-FT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid

    #105448

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Has Service

    AAV9

    Insert

    ChrimsonR-mRuby2-ST
    

    Use

    AAV

    Tags

    mRuby2 and soma targeting motif

    Mutation

    K176R in Chrimson

    Promoter

    hSyn

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLR5-CAG-NLS-SNAP

    Plasmid

    #174879

    Purpose

    PiggyBac transposon vector for expression of NLS-SNAP

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    NLS-SNAP
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. LOV-Turbo-V5_pRS415

    Plasmid

    #199667

    Purpose

    expresses LOV-Turbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Tags

    V5

    Expression

    Yeast

    Promoter

    Gal1,10

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TRE-UBC-H2-Kb SCT-T2A-GFP

    Plasmid

    #205454

    Purpose

    lentiviral plasmid for expression of mouse H2-Kb SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    H2-Kb SCT (H2-K1 Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDONR221_SLC22A2

    Plasmid

    #131964

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A2 (SLC22A2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pKanCMV-mRuby3-10aa-H2B

    Plasmid

    #74258

    Purpose

    Mammalian expression of N-terminal fusion of mRuby3 to H2B.

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    H2B
    

    Tags

    mRuby3

    Expression

    Mammalian

    Promoter

    CAG and CMV

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Barcode backbone

    Plasmid

    #158665

    Purpose

    This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    hcRED

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLYS5-MCU-Flag

    Plasmid

    #50054

    Purpose

    Expression of human MCU in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MCU-Flag (MCU Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMRXIB-mRuby3-STX17TM

    Plasmid

    #221744

    Purpose

    Stable expression of mRuby3-STX17TM in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    STX17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-228

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Promoter

    TRE3G

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcpBpaRS

    Plasmid

    #196484

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Expression

    Mammalian

    Promoter

    CMV (synthetase), Pol III tRNA promoter (tRNA)

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMRX-IP/SECFP-hATG9A

    Plasmid

    #58995

    Purpose

    Expresses SECFP-hATG9A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 9A (PPARG Human)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAL175

    Plasmid

    #22276

    Purpose

    Expresses YFP-PIF for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

    Plasmid

    #177182

    Purpose

    plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    pegRNA targeting the PEAR-GFP plasmid
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CMV-mEGFP-PKCa-C1

    Plasmid

    #112264

    Purpose

    ITRACKalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase C alpha (PRKCA Bovine)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC57 APEX

    Plasmid

    #40306

    Purpose

    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APEX (Synthetic)
    

    Use

    Unspecified

    Mutation

    K14D, W41F, E112K (relative to wild-type)

    Available Since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-Trak2

    Plasmid

    #127622

    Purpose

    N-terminal GFP-tagged mouse Trak2

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak2 (Trak2 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Nanog-2A-mCherry

    Plasmid

    #48680

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Nanog (Nanog Mouse)
    

    Use

    CRISPR

    Tags

    Cherry

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBAD-mNectarine

    Plasmid

    #21717

    Purpose

    Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

    Insert

    mNectarine fluorescent proteins
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTight-9-124

    Plasmid

    #31874

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBabe-Puro-RhoA FLARE.sc Biosensor

    Plasmid

    #12602

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge