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Showing: 3141 - 3160 of 4184 results

  4. pcDNA5-LAP-KNL1-M3

    Plasmid
    #115896
    Purpose
    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.
    Depositor
    Geert Kops
    Article
    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.
    Insert
    KNL1-M3 (KNL1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Fragment of KNL1 containing three KNL1 repeat seq…
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    115896-plasmid-map-sequence-id-224847

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  6. F2R-DuET

    Plasmid
    #213234
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    F2R (F2R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213234-plasmid-map-sequence-id-430897

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  11. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227326-plasmid-map-sequence-id-452114

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  12. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid
    #227325
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227325-plasmid-map-sequence-id-452110

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  14. pcDNA3-Endo-INPP5E

    Plasmid
    #236005
    Purpose
    Endosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    Endo-INPP5E (INPP5E Human, Synthetic)
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236005-plasmid-map-sequence-id-473813

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  15. TFORF1024

    Plasmid
    #144268
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXI3 (FOXI3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144268-plasmid-map-sequence-id-391493

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  16. Synapsin sHRPb-NLG in FSW lentiviral vector

    Plasmid
    #73148
    Purpose
    Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPb-NLG (Nlgn1 Mouse, Synthetic)
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73148-plasmid-map-sequence-id-134528

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  17. pCEP4-M2L

    Plasmid
    #20926
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Inserts
    MYC (MYC Human)
    LIN28 (LIN28A Human)
    Use
    Episomal/ebna
    Promoter
    CMV and IRES
    Available Since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20926-plasmid-map-sequence-id-9762

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  20. TFORF0497

    Plasmid
    #143707
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EGR3 (EGR3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    143707-plasmid-map-sequence-id-409085

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Showing: 3141 - 3160 of 4184 results