159,284 results

1. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX303-ZIM3-KRAB-dCas9

   Plasmid

   #154472

   Purpose

   Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYCDuet-1_Ec_rpoZ

   Plasmid

   #128837

   Purpose

   E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

   Depositor

   Seth Darst

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Insert

   rpoZ (rpoZ E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-TAZ

   Plasmid

   #32839

   Depositor

   Kunliang Guan

   Article

   Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet30b-7M SrtA

   Plasmid

   #51141

   Purpose

   expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Insert

   S.aureus SrtA 7M
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, E105K, E108Q, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB433

   Plasmid

   #74824

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-IRES-GFP II (pMIG II)

   Plasmid

   #52107

   Purpose

   a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPRyl_AXP

   Plasmid

   #84608

   Purpose

   Introduces DSB at AXP locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GW1-PercevalHR

   Plasmid

   #49082

   Purpose

   Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-ABE7.10

    Plasmid

    #102919

    Purpose

    expresses ABE7.10 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.10
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXJ-Myc-IRF1

    Plasmid

    #232641

    Purpose

    transient expresses IRF1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    IRF1 (IRF1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-blast

    Plasmid

    #26655

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. hAAVS1 TALEN Left

    Plasmid

    #52341

    Purpose

    TALEN vector for genomic targeting

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    AAVS1 TALEN Left
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. 10TALE_Pmin_fLuc

    Plasmid

    #160663

    Purpose

    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    10TALE_Pmin_fLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 hygro

    Plasmid

    #24150

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

    Depositor

    Bob Weinberg

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. hAAVS1 TALEN Right

    Plasmid

    #52342

    Purpose

    TALEN vector for genomic targeting

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    AAVS1 TALEN Right
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3xnls-mScarlet3_N1

    Plasmid

    #189775

    Purpose

    Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    3xnls-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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