Addgene

We narrowed to 32,186 results for: IND

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pBabe HAII RLF CAAX

Plasmid

#12592

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

RLF-CAAX (Rgl2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pMK389 (mCherry-BSD)

Plasmid

#121190

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mCherry-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLN593 (FuGW-S(MAFK)p-mKate2)

Plasmid

#105205

Purpose

synthetic promoter MAFK drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-Flag

Plasmid

#67164

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

FLag

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pSW596 - mTurq2

Plasmid

#115985

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mTurquoise2

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-3.2mDelta13

Plasmid

#13560

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, S112A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP-dCas9-mRFP

Plasmid

#102933

Purpose

Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

Depositor

Sebastian Bultmann

Article

Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

Insert

dCas9

Use

CRISPR

Tags

GBP (GFP binding protein) and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mMcl-1

Plasmid

#32980

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
TtGNN-GFP-empty

Plasmid

#106380

Purpose

dox inducible expression of GFP with neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pKS044

Plasmid

#65472

Purpose

pTrc plasmid expressing clpP

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

clpP (clpP )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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AIRAP 8: pCEFL.PSMB2.GST

Plasmid

#21798

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMB2 (Psmb2 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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plko-puromycin-shTRX1-259

Plasmid

#110921

Purpose

To knockdown Thioredoxin-1

Depositor

Priyamvada Rai

Article

Young et al Biochem Biophys Res Commun. 2010 Feb 12;

Insert

TRX1 (TXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

unknown

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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PonA-BI-Gl-WT-18xPP7-WT-24xMS2 (Switch)

Plasmid

#189793

Purpose

Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (Hbb Human, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ponasterone A inducible

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PH-GAP1IP4BP

Plasmid

#20200

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 19. ():.

Insert

PH-GAP1IP4BP (RASA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Isolated PH/Btk domain

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pTK101

Plasmid

#59561

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-SENP3-C526A

Plasmid

#115522

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

Insert

SENP3-C526A (Senp3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C526A

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAK02

Plasmid

#19001

Depositor

Jay Keasling

Article

Khlebnikov et al J Bacteriol. 2000 Dec . 182(24):7029-34.

Insert

arabinose transporter araE under control of Ptac (araE E. coli)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FKBP-mTagBFP2-MCS

Plasmid

#178864

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pK3FS-GAL1

Plasmid

#85778

Purpose

N-ICE plasmid pK3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJK148-Pnmt41-GBP-mCherry(N)-leu1+

Plasmid

#89070

Purpose

genomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Mutation

Promoter

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-10u

Plasmid

#13538

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207R in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO3_F

Plasmid

#146316

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Use

Tags

Expression

Mammalian

Mutation

T25A

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_TALEN-FokI(WT)-BB_V3

Plasmid

#42588

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

TALEN-FokI(WT)-BB

Use

AAV; Adeno-associated virus ()

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

CAG hybrid

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSFI-p110γ

Plasmid

#34892

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110γ (PIK3CG Human)

Use

Adaptor plasmid

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pGSTag DLC1

Plasmid

#24267

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1 Human)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pBS-SLBP

Plasmid

#45082

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2014 Jul;141(13):2592-601.

Insert

Stem Loop Binding Protein

Use

RNAi; In vitro transcription

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

May 29, 2013

Availability

Academic Institutions and Nonprofits only
pVW284

Plasmid

#139494

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-GFPenvy tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVW300

Plasmid

#139495

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pTEF PP7∆FG-GFPenvy tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pTEF

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Beclin-HA S-A

Plasmid

#46994

Purpose

Expresses Beclin1 in mammalian cells

Depositor

Kunliang Guan

Article

Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

Insert

becn1

Use

Tags

HA

Expression

Mammalian

Mutation

S14A

Promoter

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2B -376/+64

Plasmid

#24945

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

Available since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pJOG140

Plasmid

#105399

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

PKI nuclear export signal

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG141

Plasmid

#105400

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

SV40 nuclear localization signal

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Smad3 C

Plasmid

#12634

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 C (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C segment of Smad3 (aa 199-425)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRSET FLIPglu-2uDelta13

Plasmid

#13561

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F D152A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, D152A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS012

Plasmid

#65465

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

Ntag-mCherry

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

pl-TetO

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
HA-RASSF1C

Plasmid

#1981

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

RASSF1C (Rassf1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pCMP-APr

Plasmid

#61184

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtPT4

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p6661 MSCV-IP N-HA only 16E6

Plasmid

#42603

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
HA-Nore1

Plasmid

#1979

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

Nore1 (Rassf5 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
p144 pSV-EGFP/beta-gal

Plasmid

#8386

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Use

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFAST NPC1(NTD)-3Q

Plasmid

#25772

Depositor

Johann Deisenhofer

Article

Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

Insert

npc1 (NPC1 Human)

Use

Tags

His

Expression

Insect

Mutation

aa23-252 only; N70Q N122Q N185Q

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE-1m

Plasmid

#13540

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK388 (mClover-BSD)

Plasmid

#121189

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mClover-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6Rosa-CAG-Cas9

Plasmid

#118998

Purpose

Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Use

Tags

Expression

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pH7m34GW_RcCHAD-mCherry

Plasmid

#127784

Purpose

RcCHAD-mCherry expression in plants

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

Ubi10p:RcCHAD-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

Ubiquitin10 promoter + 5' UTR

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Pdar

Plasmid

#24786

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Pdar

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2011

Availability

Academic Institutions and Nonprofits only

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