We narrowed to 31,489 results for: REP

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pmCherry-C1-pmDH

Plasmid

#67897

Purpose

Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-pmDH

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CD86-mEos2

Plasmid

#98284

Purpose

mammalian expression of CD86-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CD86 (CD86 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mVenus-MKL2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBS1479-HTP-KanMX6

Plasmid

#154085

Purpose

For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

Depositor

Lidia Vasiljeva

Article

Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

Insert

his6-TEV-protein A-KanR

Use

Pcr-based tagging in yeast

Tags

his6-TEV-protein A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-LAMP1-EGFP

Plasmid

#169003

Purpose

Express rat LAMP1-EGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

Insert

rat LAMP1-EGFP

Use

Unspecified

Tags

EGFP

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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Cathepsin S

Plasmid

#84694

Purpose

for expression of Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Promoter

unknown

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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PARP2-c098

Plasmid

#216873

Purpose

Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD1

Plasmid

#172572

Purpose

For gateway cloning of N-terminal tagged MACROD1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Gateway cloning

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD2

Plasmid

#172573

Purpose

For gateway cloning of N-terminal tagged MACROD2 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Gateway cloning

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2-cmlc2-Trpa1b-2A-EGFP

Plasmid

#106405

Purpose

For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-EGFP

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pZY102

Plasmid

#73933

Purpose

This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Inserts

bar
GUS

Expression

Plant

Mutation

intron is inserted into the GUS coding region

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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PB-eDIO-EYFP

Plasmid

#104388

Purpose

PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

Depositor

Volker Busskamp

Article

Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

Insert

Enhanced Yellow Fluorescent Protein

Use

Cre/Lox; Piggybac

Expression

Mammalian

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_WTDHFR_WTTS

Plasmid

#91226

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MDO1-c014

Plasmid

#173103

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD1 (MACROD1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S58-A325, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-NFAT5

Plasmid

#79506

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

NFAT5 (NFAT5 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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