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Plasmid#62169PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloningDepositorInsertLuciferase
UseLuciferase; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L1-L4
Plasmid#62136PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA R4-R3
Plasmid#62137PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L5-L2
Plasmid#62139PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40-hCas9 L3-L2
Plasmid#62133PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloningDepositorInserthuman codon optimized Cas9
UseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40-hCas9 L5-L2
Plasmid#62134PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloningDepositorInserthuman codon optimized Cas9
UseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV-hCas9 R4-R3
Plasmid#62132PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloningDepositorInserthuman codon optimized Cas9
UseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEF-S15-FRB-Bla1
Plasmid#190916PurposeProtein fragment complementationDepositorInsertS15-FRB-Bla1 fusion protein
UseTagsN-terminal fragment of BLA fused to c-Src and FRB…ExpressionMammalianMutationPromoterCEFAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB.004
Plasmid#228670PurposeRecombination to BV DNA + SpecR (Tn7, SpecR)DepositorInsertTn7L, SpecR
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFTK094-LM-AMA002.0
Plasmid#171366PurposeEntry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SC
Plasmid#224552PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsTwin-Strep-HAExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCGN H-Ras 61L
Plasmid#14720DepositorInsertH-ras (HRAS Human)
UseTagsHAExpressionMammalianMutation61LPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJW3.42
Plasmid#62866PurposeYeast two-hybrid bait vector expressing LexA-PPTR-1 fusionDepositorInsertPPTR-1/pLexA-N bait
UseTwo-hybrid bait vectorTagsLexAExpressionYeastMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUG-P2A-tC3-W
Plasmid#129475Purpose3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3DepositorInserttruncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin CAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP-HF
Plasmid#157735Purposemammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP-HF
UseTagsHis8-FlagExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZMU-Dest
Plasmid#105089PurposeYeast expression vector for Gateway cloning and SUS bridge assaysDepositorTypeEmpty backboneUseTagsmycExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIOM15
Plasmid#20869DepositorInsertaphA::aadA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCGN K-Ras 12V, 188L
Plasmid#14722DepositorInsertK-ras (KRAS Human)
UseTagsHAExpressionMammalianMutation12V, 188LPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
42 pCOLI_K_C_HIS
Plasmid#160185PurposeC-term 8xHis uncleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags8xHisExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL
Plasmid#110741PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of nuclear lacz reporterTagsNuclear localization signalExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2
Plasmid#186361PurposeEntry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.DepositorInsertHuman Ensconsin (MAP7 Synthetic, Human)
UseExpression of a fluorescent microtubule markerTags3xEGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE H-Ras 61L, 186S
Plasmid#14719DepositorInsertH-ras (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation61L, 186SPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCGN H-Ras 61L, 189L
Plasmid#14721DepositorInsertH-ras (HRAS Human)
UseTagsHAExpressionMammalianMutation61L, 189LPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABE H-Ras 12V, 189L
Plasmid#14718DepositorInsertH-ras (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V, 189LPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAnc80L65AAP
Plasmid#92307PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAPDepositorInsertAncestral AAV Capsid Anc80L65AAP
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterRep2Available sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV_AA089
Plasmid#216105PurposegRNA expression for Cas12a (guide only)DepositorInsertgRNA expression
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_C99_sfGFP
Plasmid#207802PurposeYeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4DepositorInsertSuc2SP-C100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch3_sfGFP
Plasmid#207864PurposeYeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch3_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1-L24_sfGFP
Plasmid#207865PurposeYeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1_sfGFP
Plasmid#207801PurposeYeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterTEFAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-155
Plasmid#185939PurposeHimar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein geneDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-157
Plasmid#185940PurposeHimar transposon delivery plasmid that delivers a green fluorescent mClover3 protein geneDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
38 pCOLI_K_ C_STREP
Plasmid#160266PurposeC-term 2xStrepII uncleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepIIExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-PURO
Plasmid#58246PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selectionDepositorInsertPuro(R)
UseLentiviral and RNAi; Gateway destionation vectorTagsTetR-NLS-T2AExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only