-
Plasmid#83535PurposeConstitutive TEF1 promoter mediated expression of the UBiC geneDepositorInsertpTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-ANME2bHR1 MCR-NG
Plasmid#192774PurposeExpresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME1G37 MCR-NG
Plasmid#192773PurposeExpresses NG-tagged ANME1G37 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
groSL-GFP
Plasmid#109389PurposeGFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpgroSL-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PIP2
Plasmid#60937PurposeFluorescent FPX sensor for PIP2DepositorInsertPIP2 dimerization dependent sensor RA+B monomers
UseTagsdimerization dependent fluorescent protein monome…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RA-NES
Plasmid#61019PurposeddRFP A-NESDepositorInsertddRFP A
UseTagsHindIII site and NES sequence MNLVDLQKKLEELELDEQQExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVC
Plasmid#175063Purposeexpresses mVenus and mCherry pair in mammalian cellsDepositorInsertmVenus + mCherry
UseTagsExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
M3D395A-M14-dCas9
Plasmid#134780PurposeExpression of fusion protein M3D395A-M14-dCas9 in Mammalian cellsDepositorInsertMETTL3/METTL14 methyltransferase domain and inactive dCas9
UseTagsExpressionMammalianMutationD395APromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-GFE3
Plasmid#79869PurposeExpresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.DepositorInsertGFP-GFE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-GFP
Plasmid#66844PurposeTet-inducible expression of GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKA-207I10
Plasmid#79845PurposeExpresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoterDepositorInsertNLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
UseTagsExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-mBFP
Plasmid#112682PurposeExpression of NADPH-dependent blue fluorescent proteinDepositorInsertHis-mBFP
UseTagsHis tagExpressionBacterialMutationPromoterT5Available sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)
Plasmid#174526PurposeDual-fluorescence reporter for quantifying PylT(UAG) decoding efficiencyDepositorInsertPylS
UseTagsFlagExpressionMammalianMutationPromoterEF-1αAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV MN (eGFP)
Plasmid#89932PurposeExpresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorInsertMN
UseTagsHAExpressionMammalianMutationM.SssI residues 2-272 fragmentPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-104
Plasmid#79842PurposeExpresses NLS-PpsR2-VP16 under CMV promoterDepositorInsertNLS-PpsR2-VP16
UseTagsNuclear localization signal of nuclear cap-bindin…ExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CaYin1 NES
Plasmid#33318DepositorInsertCaYin1 NES
UseTagsNES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K840R
Plasmid#204418PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
UseTagsHA and YFPExpressionMammalianMutationLysine 840 to Arginine (K840R)PromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K1097R
Plasmid#204417PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
UseTagsHA and YFPExpressionMammalianMutationLysine 1097 to Arginine (K1097R)PromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174529PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertsPylS
PylT
UseTagsFlagExpressionMammalianMutationPromoterU6Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-E50 MCR-NG
Plasmid#192778PurposeExpresses NG-tagged E50 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME2cLM014 MCR-NG
Plasmid#192777PurposeExpresses NG-tagged ANME2cLM014 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME2cE20 MCR-NG
Plasmid#192775PurposeExpresses NG-tagged ANME2cE20 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME1BS MCR-NG
Plasmid#192772PurposeExpresses NG-tagged ANME1BS MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174528PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertPylS
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRTagsExpressionBacterialMutationPromoterPmAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
p415 Glo3 R59K S389,398 D
Plasmid#112657PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398D
UseTagsExpressionYeastMutationR59K, mutations (S389D, S398D) in Glo3 motif, pho…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHBA03
Plasmid#83537PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genesDepositorInsertpTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GA-NES
Plasmid#61018PurposeddGFP A-NESDepositorInsertddGFP A
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GB-NES
Plasmid#61017PurposeddGFP B-NESDepositorInsertddGFP B
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNA motif plasmid cloning backbone
Plasmid#107253PurposeRNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kbDepositorInsertRNA motif plasmid cloning backbone
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 4
Plasmid#99319PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-MbPylRS
Plasmid#217361PurposeExpresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmidDepositorInsertM. barkeri pyrrolysyl-tRNA synthetase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-RandE3
Plasmid#79870PurposeExpresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.DepositorInsertGFP-RandE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Twitch-1 pcDNA3
Plasmid#49812PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-1
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector
Plasmid#99292PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcPylRS(Y384F Y306A)
Plasmid#196486PurposeTo express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.DepositorInsertpyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Twitch-4 pcDNA3
Plasmid#49533PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-4
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BFP
Plasmid#106282PurposeA version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.DepositorInsertCMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2C pcDNA3
Plasmid#104501Purposefluorescent reporter for calcium signalingDepositorInsertTwitch-2C
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-AthHEN1
Plasmid#104980PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cellsDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterActin5CAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only