Skip to main content
Addgene
Showing: 751 - 800 of 6893 results

  15. Ef1a-DIO-Aion-citrine

    Plasmid
    #192571
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    Aion
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    192571-plasmid-map-sequence-id-382820

    Enlarge

  16. Ef1a-DIO-somAion-citrine

    Plasmid
    #192573
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somAion
    Use
    AAV
    Tags
    Citrine - soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192573-plasmid-map-sequence-id-379368

    Enlarge

  22. pPD1024 EF1alpha TetON3G in TU5

    Plasmid
    #139261
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV5
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139261-plasmid-map-sequence-id-325374

    Enlarge

  23. pJM469 EF1alpha TetON3G in TU4

    Plasmid
    #139260
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV4
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139260-plasmid-map-sequence-id-325373

    Enlarge

  24. pJM422 EF1alpha TetON3G in TU3

    Plasmid
    #139259
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV3
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139259-plasmid-map-sequence-id-325372

    Enlarge

  25. pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo

    Plasmid
    #128454
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    128454-plasmid-map-sequence-id-256207

    Enlarge

  26. KA601: pMVP (L3-L2) pA; EF1a::TETa-pA

    Plasmid
    #121805
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + EF1a::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121805-plasmid-map-sequence-id-237314

    Enlarge

  33. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid
    #50461
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    HA and mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50461-plasmid-map-sequence-id-182308

    Enlarge

  37. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid
    #174665
    Purpose
    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter
    Depositor
    Eli Bar
    Inserts
    LUC2
    mCherry:T2A:Hygro
    Use
    Lentiviral and Luciferase
    Tags
    mCherry and hygromycin dual reporter gene linked …
    Expression
    Mammalian
    Mutation
    Promoter
    Human cytomegalovirus immediate early enhancer/pr…
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174665-plasmid-map-sequence-id-344886

    Enlarge

  38. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid
    #170186
    Purpose
    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing
    Depositor
    Bradley Bernstein
    Article
    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:
    Insert
    CreERT2-T2A-eBFP2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170186-plasmid-map-sequence-id-336799

    Enlarge

  41. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

    Plasmid
    #50460
    Purpose
    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50460-plasmid-map-sequence-id-182307

    Enlarge

  47. Lenti-UbC-LARGE1-EF1a-BSD

    Plasmid
    #205151
    Purpose
    Express UbC-driven LARGE1 and EF-1a driven BSD
    Depositor
    Monkol Lek
    Insert
    LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205151-plasmid-map-sequence-id-405996

    Enlarge

Showing: 751 - 800 of 6893 results