We narrowed to 29,740 results for: REP

1. pDEST40-2XFL-Src-S4-S32-KD

   Plasmid

   #140299

   Purpose

   Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   lacks the UD region

   Promoter

   CMV

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIP GFP-MOSPD2 RD/LD

   Plasmid

   #186468

   Purpose

   Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R404D/L406D (mutant unable to bind FFAT motifs)

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CMV-hXRCC1-mCherry-Hygro

   Plasmid

   #176532

   Purpose

   mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

   Insert

   X-Ray Repair Cross Complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PXPR007 sgCIC-1

   Plasmid

   #74953

   Purpose

   Cas9 + sgCIC-1 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   CIC (CIC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTet-O-E2F7-T2A-PuroR

   Plasmid

   #162348

   Purpose

   Lentiviral expression of E2F7 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-mitf

   Plasmid

   #69801

   Purpose

   Used to make mitf knockout porcine cell line

   Depositor

   Jianguo Zhao

   Article

   Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

   Inserts

   mitf-sgRNA
   SpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta-Actin and U6

   Available Since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

   Plasmid

   #101300

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-509, 670-698, and 699-906 …

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HER3-DMVF-181-R

   Plasmid

   #87621

   Purpose

   C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   HER3-DMVF181-C (ERBB3 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-4EBP1 F113A

   Plasmid

   #81122

   Purpose

   expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

   Insert

   4EBP1 F113A (Eif4ebp1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F113A mutation

   Promoter

   CAG

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/CIP2A(1-905) L533E V5 His

    Plasmid

    #119288

    Purpose

    CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    L533E

    Promoter

    T7

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-VTKD1

    Plasmid

    #170846

    Purpose

    AAV backbone for transgene expression in cortical interneurons under the control of the Dlx enhancer.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-WT-ATPIF1

    Plasmid

    #85403

    Purpose

    Retroviral vector expressing wild type human ATPIF1

    Depositor

    David Sabatini

    Article

    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

    Insert

    ATPIF1 (ATP5IF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pIE-TGFB2-H6

    Plasmid

    #128500

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (TGFB2 Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

    Plasmid

    #136514

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    Codon-optimized AcrIIA5
    

    Use

    Lentiviral

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-mCherry-C1-iChR2++

    Plasmid

    #98172

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChR2++ gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 shETV5-B

    Plasmid

    #74978

    Purpose

    shETV5 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV5 (ETV5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR4-Cas9-V5

    Plasmid

    #87905

    Purpose

    Cloning vector

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    Cas9-V5
    

    Use

    Cloning vector

    Tags

    V5

    Expression

    Mutation

    Human codon optimized

    Promoter

    V5

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-hGH

    Plasmid

    #182411

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-hGH (GH1 Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.5 shETV5-A

    Plasmid

    #74977

    Purpose

    shETV5 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV5 (ETV5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLL4 sgRNA(MS2)-MS2-hA3a

    Plasmid

    #112130

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. FLAG-FullLength-TET1-CXXC(24)

    Plasmid

    #124398

    Purpose

    Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    FLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    CXXC zinc finger deletion (aa 585-624)

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA8-Flag-LARS(1-976aa)

    Plasmid

    #139689

    Purpose

    Expresses N-teminal Flag tagged aa 1-976 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-FLEXfrt-SaCas9-U6-sgGria1

    Plasmid

    #124867

    Purpose

    Mutagenesis of Gria1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria1 (Gria1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.2-GJA1-20K-V5

    Plasmid

    #49859

    Purpose

    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    GJA1-20k (GJA1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    GJA1-20k fragment corresponds to residues 213-382…

    Promoter

    CMV

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pDest-636

    Plasmid

    #159574

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    His6-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

    Plasmid

    #87906

    Purpose

    sgRNA targeting GFP

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgGFP2, sgGFP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. GFP scFv [N86/8]

    Plasmid

    #199429

    Purpose

    Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingGFP (Aequorea victoria)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

    Plasmid

    #105036

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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