We narrowed to 15,793 results for: grna

1. Icam 2 SaCas9 YAP sgRNA2

   Plasmid

   #99735

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. DNA-PKcs N-terminal sgRNA

   Plasmid

   #207093

   Purpose

   pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AGCGGGACTCGGCGGCATGG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SMVP-Cas9N-U6-gRNA M3

   Plasmid

   #80936

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

   Plasmid

   #71828

   Purpose

   Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   U6

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

   Plasmid

   #154197

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC2-A, sgRNA-BC2-C
   

   Use

   Lentiviral; Catch barcode activation

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and mU6

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

   Plasmid

   #154002

   Purpose

   Empty SgRNA expression system matching CasRx

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   SgRNA expression system
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6, EF1a

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR v2 puro hGSDME gRNA1

   Plasmid

   #223519

   Purpose

   knocking out hGSDME in human cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

   Plasmid

   #105043

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stk11 (Stk11 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

   Plasmid

   #218529

   Purpose

   sgRNA targeting human EIF2AK1/HRI

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK1 (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB700 lenti gRNA EYFP

    Plasmid

    #167906

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6-(BbsI)sgRNA_CAG-Venus-bpA

    Plasmid

    #86985

    Purpose

    For cloning and expression of sgRNA together with Venus expression

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Venus GFP variant

    Promoter

    CAG

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLb-Cpf1-pGL3-U6-sgRNA

    Plasmid

    #107682

    Purpose

    Expresses LbCpf1 crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9S(RBSmut)_Psyn-sgRNA500

    Plasmid

    #149576

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHKMC1: Empty sgRNA for Cloning

    Plasmid

    #67720

    Purpose

    Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

    Depositor

    Monica Colaiacovo

    Article

    Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    PU6

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-evoCjCas9-ABE8e_gRNA

    Plasmid

    #202561

    Purpose

    hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid

    #221503

    Purpose

    CRISPRi machinery fish codon optimized expressing plasmid

    Depositor

    Alessandro Ori

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    D10A and H839A point mutations

    Promoter

    Zebrafish Ubiquitin promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

    Plasmid

    #211966

    Purpose

    Express gRNA against JARID2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting JARID2 (JARID2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

    Plasmid

    #83947

    Purpose

    gRNA cassette-carrying vector with natMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    gRNA targeting ADE2 gene
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-PpU6P-sgRNA-L3L2

    Plasmid

    #113740

    Purpose

    Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-PpU6P-sgRNA-L1L4

    Plasmid

    #113736

    Purpose

    Gateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102797

    Purpose

    Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H231L

    Promoter

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pZ148-BhCas12b-sgRNA-scaffold

    Plasmid

    #122448

    Purpose

    Expresses sgRNA for BhCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BhCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSB700 lenti gRNA Blasto_zhang2.0

    Plasmid

    #167912

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-SMVP-Cas9N-U6-gRNA P1

    Plasmid

    #80943

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-U6-gRNA-TnT-Cre

    Plasmid

    #132551

    Purpose

    For loss-of-function experiments in cardiomyocytes

    Depositor

    William Pu

    Inserts

    TnT-Cre
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TnT and U6

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDM002-sgRNA-lenti-ZeoR

    Plasmid

    #200060

    Purpose

    Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    Bleomycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLentiCRISPRv2-TRAF6-targeting sgRNA 1

    Plasmid

    #131345

    Purpose

    gRNA targeting mouse TRAF6

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    gRNA targeting mouse TRAF6 (Traf6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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