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Showing: 6361 - 6400 of 159111 results

  2. CCR10-DuET

    Plasmid
    #213197
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR10 (CCR10 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213197-plasmid-map-sequence-id-430761

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  3. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

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  15. mEmerald-Lifeact-7

    Plasmid
    #54148
    Purpose
    Localization: Actin, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. TFORF2862

    Plasmid
    #143645
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SREBF1 (SREBF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143645-plasmid-map-sequence-id-400473

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  20. pDONR221-SLCO1B1_STOP

    Plasmid
    #161354
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLCO1B1 (SLCO1B1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161354-plasmid-map-sequence-id-351777

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  21. PS-CFP2-TOMM20-N-10

    Plasmid
    #57250
    Purpose
    Localization: Outer Mitochondrial Membrane, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 13, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. EDNRA-DuET

    Plasmid
    #213232
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    EDNRA (EDNRA Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213232-plasmid-map-sequence-id-430792

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  27. SpCas9-NRCH-ABE8e(V106W)

    Plasmid
    #198556
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198556-plasmid-map-sequence-id-388914

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  28. EF1a-mito-dsRED2

    Plasmid
    #174541
    Purpose
    mitochondria targeted dsRED expressed off of EF1a promoter
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Use
    Lentiviral
    Tags
    dsRED
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174541-plasmid-map-sequence-id-345057

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  31. pQE-BR1

    Plasmid
    #160333
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    BR1
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160333-plasmid-map-sequence-id-326604

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  32. pCAGG-NFIX1

    Plasmid
    #112702
    Purpose
    Expression of NFIX1 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I X1 (Nfix Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112702-plasmid-map-sequence-id-212396

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  36. CXCR5-DuET

    Plasmid
    #213222
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR5 (CXCR5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213222-plasmid-map-sequence-id-430784

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  37. pOpen_taq

    Plasmid
    #153315
    Purpose
    Bacterial expression of Taq polymerase
    Depositor
    Drew Endy
    Insert
    Taq polymerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153315-plasmid-map-sequence-id-298774

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  39. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

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Showing: 6361 - 6400 of 159111 results