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We narrowed to 7,509 results for: PAC

Showing: 6401 - 6440 of 7509 results

  8. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid
    #74709
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pJW1354

    Plasmid
    #69490
    Purpose
    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms
    Depositor
    Jordan Ward
    Article
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    Use
    CRISPR; Template to generate cassettes for -media…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69490-plasmid-map-sequence-id-122661

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  20. TR-Csy4-H29A

    Plasmid
    #80602
    Purpose
    Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    Csy4-H29A
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    His29 mutated to Ala
    Promoter
    CBA
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    80602-plasmid-map-sequence-id-152080

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  25. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  29. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

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  30. pRPa-iSL(MCS1+2)

    Plasmid
    #69244
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69244-plasmid-map-sequence-id-124145

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  31. pCS4

    Plasmid
    #55751
    Purpose
    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-59 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55751-plasmid-map-sequence-id-86408

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  32. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  33. pYFAC-CH1

    Plasmid
    #168977
    Purpose
    AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in fil…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    168977-plasmid-map-sequence-id-359562

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  35. cPE

    Plasmid
    #183549
    Purpose
    Expresses compact Prime Editor in mammalian cells
    Depositor
    Wen Xue
    Article
    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do
    Insert
    SpCas9 nickase (H840A), Reverse Transcriptase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183549-plasmid-map-sequence-id-365389

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Showing: 6401 - 6440 of 7509 results