We narrowed to 9,202 results for: sacs

1. p415 Glo3

   Plasmid

   #112651

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Met25

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS426-GFP-Osh1-PH

   Plasmid

   #71372

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh1-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa267-388

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pGEX2TK-Skm1-PH

   Plasmid

   #71371

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Skm1-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa1-132

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pJAT23

   Plasmid

   #204312

   Purpose

   For generating targeting inversions. C-terminal split GFP, expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-GFPc
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b I-SceI (Ctermhis -MH)

   Plasmid

   #195613

   Purpose

   Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHES822

   Plasmid

   #89193

   Purpose

   Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pZ-YFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pZ

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCfB2337

   Plasmid

   #67555

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX4T1-GST-eIF4G1-His6

   Plasmid

   #37231

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   TIF4631 (TIF4631 Budding Yeast)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Sc eRF1 (WT)

   Plasmid

   #175413

   Purpose

   Encodes His-MBP-TEV-WT eRF1

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF1 (SUP45 Budding Yeast)
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMyc0ElbLuc

    Plasmid

    #53242

    Purpose

    reporter plasmid without myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMycOElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRS423-TyrSgH

    Plasmid

    #163973

    Purpose

    Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Dcp2 FL

    Plasmid

    #163574

    Purpose

    express wild type Dcp2 at endogenous level in S.cerevisiae

    Depositor

    Roy Parker

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    DCP2 promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Dhh1-EGFP

    Plasmid

    #201418

    Purpose

    Bacterial protein expression of Dhh1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMAL724

    Plasmid

    #169151

    Purpose

    Encodes OptoQ-AMP5

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS_KS_attB2_SA(0)_T2AGAL4

    Plasmid

    #125211

    Purpose

    Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG416GPD-Cerulean-GOS1

    Plasmid

    #18846

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    GOS1 (GOS1 Budding Yeast)
    

    Use

    Tags

    cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pYES2.1-pomSir1

    Plasmid

    #107447

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Sir1 (sir1 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    H523R, A750T

    Promoter

    GAL1

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS405 VAC8-GFP

    Plasmid

    #166836

    Purpose

    Expresses Vac8-GFP in yeasts.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYES2.1-cerMET6

    Plasmid

    #107438

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET6 (MET6 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBS_KS_attB2_SA(2)_T2AGAL4

    Plasmid

    #125213

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS_KS_attB2_SA(1)_T2AGAL4

    Plasmid

    #125212

    Purpose

    Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. D771 leu2::LYS2 Disruptor Converter

    Plasmid

    #51677

    Purpose

    Yeast marker swap plasmid for converting LEU2 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YIplac211-HaloTag-DRS2

    Plasmid

    #177103

    Purpose

    Expresses HaloTag-Drs2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    DRS2 (DRS2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Mammalian_SaAID

    Plasmid

    #188653

    Purpose

    Expresses SaAID in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nSaCas9-AID-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    pCMV

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p416GPD-PYK1

    Plasmid

    #34604

    Depositor

    Markus Ralser

    Article

    Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

    Insert

    PYK1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. N-GFP-Dcp2

    Plasmid

    #163585

    Purpose

    express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    DCP2 promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIM690

    Plasmid

    #74638

    Purpose

    alias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG416GPD-Cerulean-SEC4

    Plasmid

    #18844

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    SEC4 (SEC4 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pMAL669

    Plasmid

    #169148

    Purpose

    Encodes OptoQ-AMP2

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge