19,634 results

1. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Expression

   Mammalian

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP Centrin2 (Nigg UK185)

   Plasmid

   #41147

   Depositor

   Erich Nigg

   Article

   Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

   Insert

   Centrin-2 (CETN2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV9retro

   Plasmid

   #224687

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   Zhigang He

   Article

   Zhang et al Cell. 2024 May 3:S0092-8674(24)00447-1.

   Insert

   Rep2/Cap9retro
   

   Use

   AAV

   Mutation

   insertion of 10-mer peptide from AAV2retro (LADQD…

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. rAAV2/cMG

   Plasmid

   #184539

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-cMG capsid
   

   Use

   AAV; Helper vector for packaging

   Mutation

   changes on AAV9 capsid: K449R, A589D; insertion o…

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBMN-I-GFP

   Plasmid

   #1736

   Purpose

   This retroviral vector has an IRES-GFP downstream of the cloning sites

   Depositor

   Garry Nolan

   Insert

   IRES-GFP
   

   Use

   Retroviral

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-MetGFP

   Plasmid

   #37560

   Depositor

   David Rimm

   Insert

   MET (MET Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hM3D(Gq)-mCherry

   Plasmid

   #50474

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-iGlucoSnFR-TS

   Plasmid

   #127952

   Purpose

   Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

   Depositor

   Gary Yellen

   Article

   Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

   Insert

   iGlucoSnFR-TS
   

   Use

   AAV

   Tags

   His6 and Xpress epitope

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5-Flag-XBP1s

   Plasmid

   #63680

   Purpose

   Mammalian expression of Flag tagged XBP1s

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   spliced

   Promoter

   CMV

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX Nup62Fv2GFP

    Plasmid

    #139683

    Purpose

    Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

    Depositor

    Edward Campbell

    Article

    Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

    Insert

    Nup62 (NUP62 Human)
    

    Use

    Lentiviral

    Tags

    2 copies of GFP and Dimerization domain FKBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ClopHensorN

    Plasmid

    #50758

    Purpose

    Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

    Depositor

    Colin Akerman

    Article

    Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

    Insert

    ClopHensorN
    

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    CAG

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CAG and TRE

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1321 HPV-16 E6/E7

    Plasmid

    #8641

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E6/E7 (E7, E6 Human papillomavirus)
    

    Expression

    Mammalian

    Available Since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-CMV-Flag-CDK13_IDG-K

    Plasmid

    #135276

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC3.1-caggs-Crimson-CAAX

    Plasmid

    #190041

    Purpose

    Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

    Depositor

    Michael Lin

    Article

    Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

    Insert

    Crimson
    

    Tags

    CAAX

    Expression

    Mammalian

    Promoter

    caggs

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS_NP_EBOV

    Plasmid

    #103049

    Purpose

    Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
    

    Expression

    Mammalian

    Mutation

    silent mutation (T to C) at position 928 of NP ge…

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEP4 E02S ET2K

    Plasmid

    #20927

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. sfGFP-N1

    Plasmid

    #54737

    Purpose

    Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Geoffrey Waldo, Michael Davidson

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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