We narrowed to 13,031 results for: Hal

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pDBTrp-LexABD-CRY2FL

Plasmid

#78210

Purpose

Use with Addgene 40615 for blue light transcription control in yeast

Depositor

Chandra Tucker

Article

Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

Insert

LexABD-CRY2 (CRY2 Mustard Weed)

Tags

lexA binding domain

Expression

Yeast

Mutation

contains endogenous CRY2 NLS

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pVP2A-PIF6

Plasmid

#73369

Purpose

Expresses Adeno-associated virus VP2 with PIF6 insert.

Depositor

Junghae Suh

Article

Gomez et al ACS Nano. 2015 Nov 30.

Insert

PIF6 (PIL2 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0915 (CRISPR with target 3)

Plasmid

#130643

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(target-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSL2669 (pTnsABf_Vch)

Plasmid

#200897

Purpose

Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsA-TnsB

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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CMV/N3IC-HA

Plasmid

#47618

Purpose

in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

Depositor

Urban Lendahl

Article

Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

Insert

Notch3 Intracellular domain (Notch3 Mouse)

Use

In vitro transcription and translation

Tags

HA

Expression

Mammalian

Mutation

codons 1664 (Met) to 2318 (Ala)

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
mRIP3 GFP RHIM mut

Plasmid

#41384

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pOME0116

Plasmid

#178392

Purpose

Expresses KSHV ORF 8 gB GQ994935 tagged

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF 8

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-HA-2xMCP-KRAB

Plasmid

#126589

Purpose

Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

2XMCP-KRAB

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-RAP2A-GFP

Plasmid

#86124

Purpose

Retroviral vector expressing RAP2A-GFP-IRES-Bsd

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

RAP2A (RAP2A Human)

Use

Retroviral

Tags

3xFLAG and GFP

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAL188

Plasmid

#22277

Purpose

Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-NDUFAF5-HA

Plasmid

#86125

Purpose

Retroviral vector expressing NDUFAF5-HA-IRES-Bsd

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

NDUFAF5 (NDUFAF5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
MSCV-puro-N3FLAG-MYB-FL

Plasmid

#105596

Purpose

retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch3 LNR

Plasmid

#188338

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch3-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_FAP site with scFv

Plasmid

#188351

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLPCX2-FLAG-Smad4

Plasmid

#89834

Purpose

Express Smad4 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

SMAD4 (SMAD4 Human)

Use

Retroviral

Tags

FLAG

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_(GGS)6_SNIPR

Plasmid

#188344

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_(GGS)6_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-PIF-SOS2cat

Plasmid

#50850

Purpose

Optogenetic Ras activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Inserts

SOS2cat (SOS2 Human)
PIF6 1-100

Use

Lentiviral

Tags

TagBFP

Mutation

residues 566–1049

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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B2094_CRY2olig-miRFP

Plasmid

#215114

Purpose

Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CRY2olig-miRFP (CRY2 Mustard Weed)

Tags

miRFP

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 PuroR L3-L2

Plasmid

#62164

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Gateway Cloning

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_FnIII-Notch

Plasmid

#188342

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_FnIII-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY139

Plasmid

#223486

Purpose

tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tph-1 (tph-1 Nematode)

Expression

Worm

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEY82

Plasmid

#191077

Purpose

flp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-7 (flp-7 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-TEM1(S70A,D179G)/csTEM1

Plasmid

#81163

Purpose

pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

Codon swapped TEM-1 from H26 to W290 and His6x

Expression

Bacterial

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

lac promoter

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-mKate2

Plasmid

#67671

Purpose

Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-P2A-mKate2

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9

Plasmid

#60551

Purpose

Expresses CibN-dCas9 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

CibN-dCas9

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Clover-pBAD

Plasmid

#54575

Purpose

Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and Clover

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pXTK066 [Con-Pro_nptII-Broad_PartType8]

Plasmid

#229303

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

nptII Promoter (Broad)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV Ef1a DIO GeNL_SS hGtACR1

Plasmid

#233511

Purpose

Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

Inserts

mNeonGreen
ISN-IE
hGtACR1

Use

AAV

Tags

Gaussia luciferase signal sequence

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-hIes6

Plasmid

#15356

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

hIes6 (INO80C Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pFB-HTB-ASK1

Plasmid

#29517

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

ASK1 (SKP1 Mustard Weed)

Expression

Insect

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_Blasto

Plasmid

#167864

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY51

Plasmid

#191054

Purpose

mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mec-3 (mec-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_CO-Mini-C

Plasmid

#190337

Purpose

Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini-C, PCSK9 +1A insertion pegRNA

Use

AAV

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only

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We narrowed to 13,031 results for: Hal