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We narrowed to 9,686 results for: Gnas

Showing: 8151 - 8200 of 9686 results

  2. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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  3. C25-pLAC

    Plasmid
    #45283
    Purpose
    lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide
    Depositor
    Gordon Laurie
    Article
    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.
    Insert
    pLAC-C25 (LACRT Human)
    Use
    Protein expression vector
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    contains aa20-113 of lacritin
    Promoter
    T7
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    45283-plasmid-map-sequence-id-63156

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  7. pFastBacDual with LFn + PrgJ

    Plasmid
    #84869
    Purpose
    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    PrgJ
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84869-plasmid-map-sequence-id-162908

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  17. PF3D7_0606800-bio

    Plasmid
    #47790
    Purpose
    Expresses enzymatically monobiotinylated full-length PF3D7_0606800 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PF3D7_0606800
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47790-plasmid-map-sequence-id-68827

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  18. pGM55

    Plasmid
    #71249
    Purpose
    luciferase reporter with hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Bert et al Plasmid. 2000 Sep;44(2):173-82.
    Insert
    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71249-plasmid-map-sequence-id-131890

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  24. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  27. pDule-Acd82

    Plasmid
    #174099
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174099-plasmid-map-sequence-id-345716

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  41. P12p-bio

    Plasmid
    #47726
    Purpose
    Expresses enzymatically monobiotinylated full-length P12p ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised P12p
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47726-plasmid-map-sequence-id-68869

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  44. RON3-bio

    Plasmid
    #47799
    Purpose
    Expresses enzymatically monobiotinylated full-length RON3 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RON3
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47799-plasmid-map-sequence-id-68821

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  45. MSP6-bio

    Plasmid
    #47732
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP6
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47732-plasmid-map-sequence-id-68561

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  47. RAP2-bio

    Plasmid
    #47795
    Purpose
    Expresses enzymatically monobiotinylated full-length RAP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RAP2
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47795-plasmid-map-sequence-id-68801

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  48. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  49. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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Showing: 8151 - 8200 of 9686 results