We narrowed to 15,793 results for: grna

1. lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

   Plasmid

   #176838

   Purpose

   To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   sgPDX1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

   Plasmid

   #149594

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. px335-sgRNA-EF(Cas9n)-Eif4a1-R

   Plasmid

   #122345

   Purpose

   Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Eif4a1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

   Plasmid

   #89061

   Purpose

   AAV-gRNA targeting the murine Ldlr gene

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHU6_chr12_FAM19A2-down-gRNA-rev

   Plasmid

   #81222

   Purpose

   pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locus

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   hU6 expression of gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-NQL007-NANOG-sgRNA

   Plasmid

   #175555

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

   Plasmid

   #163138

   Purpose

   Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PasCas13b-gRNA-EGFP(W58stop)

   Plasmid

   #180507

   Purpose

   PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   PasCas13b-gRNA targeting EGFP(W58stop)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PasCas13b-gRNA-Nluc(W12stop)

   Plasmid

   #180506

   Purpose

   PasCas13b-gRNA targeting Nluc(W12stop) for RNA editing

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   PasCas13b-gRNA targeting Nluc(W12stop)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV-gRNA-CTBP2-MGMT_1

    Plasmid

    #136419

    Purpose

    Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

    Depositor

    Massimo Squatrito

    Insert

    U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPR - Amplicon, JAK2 sgRNA 1

    Plasmid

    #70679

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. PX459v2-ILK-gRNA 1-exon 1

    Plasmid

    #163320

    Purpose

    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 1_Exon1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

13. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6F

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

14. SauCas9KKH CCR5-nicking sgRNA

    Plasmid

    #169863

    Purpose

    SauCas9KKH nicking sgRNA for CCR5

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9KKH CCR5-nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

    Plasmid

    #105019

    Purpose

    Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Vps39 (Vps39 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p426-SNR52p-gRNA.clr-2.Y-SUP4t

    Plasmid

    #89692

    Purpose

    gRNA for clr-2 locus

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for clr-2 locus
    

    Use

    CRISPR; Grna

    Tags

    No

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC-U63-tgRNA-Rev

    Plasmid

    #112811

    Purpose

    gRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in Drosophila

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

    Plasmid

    #105027

    Purpose

    Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pM117: VEGFA array presgRNA

    Plasmid

    #166869

    Purpose

    U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting array presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W

    Plasmid

    #159287

    Purpose

    Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5)

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    guide RNA targeting KAT7 (ID. 5) (KAT7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

    Plasmid

    #149656

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pX330 Mouse 5' Npm1 gRNA

    Plasmid

    #127900

    Purpose

    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC57-Sa sgRNA expression vector

    Plasmid

    #107720

    Purpose

    For in vitro transcription of Sa sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAN-PBAD-sgRNA-A2T

    Plasmid

    #62258

    Purpose

    expression of A2T sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A2T sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid

    #70660

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

26. Cas9 ROSA sgRNA mCherry

    Plasmid

    #155280

    Purpose

    Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker

    Depositor

    Martin Carroll

    Insert

    spCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

    Plasmid

    #68346

    Purpose

    Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

    Inserts

    3xgRNA;PTENA, p53B,SMAD4A
    FlPO
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. px335-sgRNA-EF(Cas9n)-Eif4a1-L

    Plasmid

    #122346

    Purpose

    Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Eif4a1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138316

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAN-PBAD-sgRNA-A1T

    Plasmid

    #62256

    Purpose

    expression of A1T sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A1T sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge