We narrowed to 9,393 results for: Coli

1. pAC-PHYT

   Plasmid

   #53300

   Purpose

   Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

   Insert

   crtE, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a-GFP-Patronin CC 868–1457

   Plasmid

   #59053

   Purpose

   for E. coli expression of Patronin CC truncation

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pENV8(GAAA)-GFPuv

   Plasmid

   #70128

   Purpose

   Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

   Depositor

   Robert Batey

   Article

   Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

   Insert

   kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a+GFP-CAMSAP1 CKK

   Plasmid

   #59031

   Purpose

   expresses CKK domain of CAMSAP1 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP1 CKK domain (CAMSAP1 Human)
   

   Use

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAtipiTrc

   Plasmid

   #53275

   Purpose

   Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

   Insert

   ipi1 (IPP2 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CoV-NSP3-c001

   Plasmid

   #173082

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E1000-K1173, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MDO2-c003

   Plasmid

   #173105

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K7-D243

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MDO2-c005

   Plasmid

   #173106

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   K7-D243

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAL1

   Plasmid

   #197285

   Purpose

   Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

   Depositor

   Bogumil Karas

   Article

   Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

   Insert

   Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTYF-2xSYN-tTA

    Plasmid

    #19982

    Depositor

    Sergey Kasparov

    Article

    Liu et al J Gene Med. 2008 May . 10(5):583-92.

    Insert

    2xSYN-tTA (SYN1 Budding Yeast, Human, E.coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

    Plasmid

    #127360

    Purpose

    gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    R118G

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIC-Bio2_SMAC(delta(55))_9His

    Plasmid

    #197976

    Purpose

    E. coli expression of SMAC(delta(55))-9His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    Smac (DIABLO Human)
    

    Use

    Tags

    9His

    Expression

    Bacterial

    Mutation

    delta (1-55)

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11-His6-Pif6-sfGFP-ssrA

    Plasmid

    #140891

    Purpose

    Expression of PIF-sfGFP-ssrA in E. coli

    Depositor

    Neal Devaraj

    Article

    Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    PIF-sfGFP-ssrA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPP378

    Plasmid

    #158797

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-1 (D371A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-28a-His6-SUMO-VAMP2

    Plasmid

    #135551

    Purpose

    Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP2
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

    Plasmid

    #127357

    Purpose

    gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    R118G

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a - GR

    Plasmid

    #89614

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi Glutathione reductase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Glutaredoxin
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSM17

    Plasmid

    #101641

    Purpose

    Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    eqFP486
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSM12

    Plasmid

    #101643

    Purpose

    Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    meffCFP
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1BR9-mCherrySTOP

    Plasmid

    #193866

    Purpose

    E. coli expression vector for N-ter GFP11 tagged mCherry.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    mCherry
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPP384

    Plasmid

    #158799

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-3 (D413A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD-BpBBS

    Plasmid

    #199467

    Purpose

    Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

    Depositor

    Vladislav Verkhusha

    Article

    Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

    Insert

    BpBBS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX4T-1-human RNF146 RING-WWE (30-183)

    Plasmid

    #132611

    Purpose

    expresses human RNF146 RING-WWE (30-183) in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

    Insert

    RNF146 RING-WWE (30–183)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BPK2013-AkCas12b

    Plasmid

    #121950

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AkCas12b
    

    Use

    Tags

    10xHis tag and 3xHA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pETM6-G6-mCherry

    Plasmid

    #66533

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter G6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - G6

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTRCHisB-TRF2_M

    Plasmid

    #50490

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Myb (TERF2 Human)
    

    Use

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 438-500

    Promoter

    pTrc

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. D. vulgaris Cas7/pHMGWA

    Plasmid

    #81184

    Purpose

    Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas7
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid

    #73220

    Purpose

    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    dCas9 from S. pyogenes
    

    Use

    Tags

    c-myc

    Expression

    Bacterial

    Mutation

    Silent mutation at bp 1341 A->C to remove an E…

    Promoter

    PpsbA2

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRAN334

    Plasmid

    #120814

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

    Insert

    cyan fluorescent protein, codon-optimized for low GC organisms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-DEST42-SARA-V5-His6

    Plasmid

    #67431

    Purpose

    Expression of hSara with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pETM6-H9-mCherry

    Plasmid

    #66532

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H9

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H9

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-28a-His6-SUMO-VAMP8

    Plasmid

    #135553

    Purpose

    Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP8
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. D. vulgaris Cas5c/pHMGWA

    Plasmid

    #81182

    Purpose

    Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas5c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. D. vulgaris Cas8c/pHMGWA

    Plasmid

    #81183

    Purpose

    Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas8c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSM14

    Plasmid

    #101645

    Purpose

    Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    efasCFP
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p3E-Dam-myc no-pA

    Plasmid

    #82600

    Purpose

    3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    Dam-Myc (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. U_(+36GFP)-Cre.ape

    Plasmid

    #71749

    Purpose

    U+36GFP-Cre fusion expression in E. Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    U_(pos36GFP)-Cre
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHL 651

    Plasmid

    #53016

    Purpose

    LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    LacIq
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pCon

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only