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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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ELF3_pet28a

Plasmid

#131658

Purpose

Bacterial expression of ETS gene ELF3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF3 (ELF3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

Plasmid

#62975

Purpose

Expresses HA-tagged rat SOX9 residues 286-507

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA (YPYDVPDY)

Expression

Mammalian

Mutation

codes for residues 286-507

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pLJC6-METAP1-3xFLAG

Plasmid

#163453

Purpose

lentiviral overexpression vector for human METAP1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

METAP1 (METAP1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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Bxb1_miRFP703

Plasmid

#183759

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

miRFP703

Tags

Lck lipidation signal

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pACT2-μ3A

Plasmid

#198174

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-3 μ3A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

silent substitution in codon 225

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgHK2_2

Plasmid

#163461

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting HK2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-mKate2

Plasmid

#67671

Purpose

Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-P2A-mKate2

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pUWR-CadTS-C

Plasmid

#58326

Purpose

Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

E-cadherin tension sensor control

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

Plasmid

#120269

Purpose

Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

eSyn-DIO-TVA950:YFP

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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mcherry(C1)_RepoMan

Plasmid

#46274

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Biol. 2006 Feb 27;172(5):679-92.

Insert

RepoMan (CDCA2 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Dppa2

Plasmid

#40799

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Dppa2 (Dppa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP(C1)-RepoMan

Plasmid

#44212

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Biol. 2006 Feb 27;172(5):679-92.

Insert

RepoMan (CDCA2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pSico-shRaptor

Plasmid

#81086

Purpose

conditional expression of an shRNA targeting murine Raptor

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Raptor shRNA (Rptor Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ELAVL1

Plasmid

#106106

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL1 (ELAVL1 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD59-T2A-GFP

Plasmid

#205451

Purpose

lentiviral plasmid for expression of mouse CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD59 (Cd59a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-RHOB

Plasmid

#182237

Purpose

Expresses RHOB GTPase with tripartite split GFP10 tag

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

ras homolog family member B (RHOB Human)

Tags

GFP10 (split-GFP tripartite)

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDSRaD-sdi1

Plasmid

#219566

Purpose

Mammalian expression of human sdi1 (p21 CDK inhibitor)

Depositor

Asao Noda, James R Smith

Article

Noda et al Exp Cell Res. 1994 Mar;211(1):90-8. doi:

Insert

sdi1 (CDKN1A Human)

Expression

Mammalian

Promoter

SRa

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras G12V Y40C

Plasmid

#12276

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-Blast

Plasmid

#44245

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

FKBP (FKBP1A Human)

Use

Lentiviral

Tags

Gal4/DBD/aa1-147 and HA

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pKC158

Plasmid

#134242

Purpose

CENP-A Halo expression construct

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP-A-Halo tagging construct (CENPA Human)

Use

Human targeting

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HS-EB3-GFP

Plasmid

#105948

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inpp5e(D477N)

Plasmid

#96809

Purpose

phosphatase-dead control for 5HT6-YFP-Inpp5e, CAAX-null

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inpp5e(D477N)

Tags

YFP

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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GABPA_pet28a

Plasmid

#131640

Purpose

Bacterial expression of ETS gene GABPA

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

GABPA (GABPA Human)

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee-Kras

Plasmid

#134777

Purpose

mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee-Kras

Tags

FLAG and mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBCMV-HaloTag-VPg(FCV)

Plasmid

#167311

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

HaloTag-VPg(FCV)

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-H2B-6

Plasmid

#55056

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

H2B (H2BC11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCX-Lyn-SponGee

Plasmid

#134776

Purpose

mammalian expression of Lyn-SponGee, a lipid raft-targeted variant of the cGMP buffer SponGee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

Lyn-SponGee

Tags

FLAG and mRFP

Expression

Mammalian

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXA10-MSCV

Plasmid

#20980

Depositor

Corey Largman

Article

Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

Insert

HOXA10 (HOXA10 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras G12V E37G

Plasmid

#12275

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
5HT6-YFP-Inpp5e

Plasmid

#96808

Purpose

cilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-null

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inpp5e

Tags

YFP

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE bleo human IRS-1

Plasmid

#11448

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

Insert

IRS-1 (IRS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
D28 Cortactin GFP

Plasmid

#26723

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion removed 28 amino acids (aa# 334-361) enc…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGoldyTALEN-VIM-TAL-2

Plasmid

#62852

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGoldyTALEN-VIM-TAL-1

Plasmid

#62855

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-PKCG

Plasmid

#16180

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

PRKC gamma (PRKCG Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pME-mKate-P2A-APEX2-NS

Plasmid

#67670

Purpose

Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-NS

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 64,611 results for: cell;