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Showing: 6721 - 6760 of 25998 results

  7. pET-Sfp R4-4 mutant

    Plasmid
    #173491
    Purpose
    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.
    Depositor
    Jun Yin
    Article
    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d
    Insert
    Sfp-R44
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173491-plasmid-map-sequence-id-349047

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  17. pGL3-LRF

    Plasmid
    #66936
    Purpose
    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).
    Depositor
    Alvaro Monteiro
    Article
    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV-40
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. lentiCRISPR - AAVS1 sgRNA

    Plasmid
    #70661
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against AAVS1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6721 - 6760 of 25998 results