-
Plasmid#153019PurposeStep-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.DepositorInsertSOUL
UseAAVTagsP2A-tdTomatoExpressionMammalianMutationC128S/D156A/T159CPromoterAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato
Plasmid#209298PurposeA lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.DepositorInsertsdCas9
tdTomato
UseLentiviralTagsExpressionMammalianMutationPromotertetracycline-inducible promoter (TRE3G)Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-tdTomato
Plasmid#73462PurposeExpresses tdTomato for monosynaptic circuit mappingDepositorInserttdTomato
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-rev-ChR2-tdtomato
Plasmid#18917PurposeCre-dependent viral expression of ChR2-tdTomato for neuronal excitationDepositorInsertChannelrhodopsin 2-tdtomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdtomatoExpressionMutationPromoterCAGAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#133786PurposeCre-dependent tdTomato fluorescent reporter for cell labelingDepositorInsertpAAV-EF1a-DIO-tdTomato-WPRE
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-TdTomato-WPRE-pA
Plasmid#37092PurposeExpresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertTdTomato
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-Flex-rev-oChIEF-tdTomato
Plasmid#30541PurposeCre-dependent viral expression of oChIEF-tdTomato for optogenetic excitationDepositorInsertoChIEF-tdtomato
UseAAV and Cre/LoxTagstdTomatoExpressionMutationPromoterCAGAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FRT-Rev-TdTomato
Plasmid#191203PurposeFlpO dependent TdTomato expressionDepositorInsertTdTomato
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-TdTomato
Plasmid#166600PurposeEncodes Cre-dependent ChR2-TdTomato under control of the TREDepositorInsertChR2-TdTomato
UseAAVTagsExpressionMutationH134RPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Chronos-tdTomato]
Plasmid#84481PurposeAAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorInsertKif5c (Kif5c Rat)
UseTagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-tdTomato-polyA
Plasmid#174022PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserttdTomato
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#130909PurposeAAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorHas ServiceAAV8InsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterCAGAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFlpStop-HDR-UAS-2.1-tdTom
Plasmid#89148PurposeFlpStop plasmid for CRISPR/HDR. Has MCS for homology armsDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoter5XUAS, 3XP3-dsRedAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-WPRE
Plasmid#51505PurposeCan be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoterDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Chronos-tdTomato]
Plasmid#84484PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ai66(RCRL-tdT) targeting vector
Plasmid#61578PurposeTarget a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertCAG-RSR-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#202610PurposeDouble floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-tdTomato-P2A-paCaMKII(K42M)
Plasmid#165433PurposeExpresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.DepositorInserttdTomato-P2A-paCaMKII(K42M)
UseTagstdTomatoExpressionMammalianMutationK42M: Kinase dead mutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112668PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Puro
Plasmid#135687PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-Bcl2-IRES-tdtomato(TET005)
Plasmid#117857PurposeThis plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.DepositorInsertBcl2 (Bcl2 Mouse)
UseTta/tetoTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128589PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorHas ServiceAAV8InsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterSynAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]
Plasmid#84483PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-iRFP670-Tdtomato-GFP
Plasmid#111817PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
Tdtomato
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-tdTomato-P2A-paCaMKII(T286A)
Plasmid#165432PurposeExpresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.DepositorInserttdTomato-P2A-paCaMKII(T286A)
UseTagstdTomatoExpressionMammalianMutationT286A: Autophosphorylation deficient mutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 tdTomato LIC cloning vector (6F)
Plasmid#86963PurposeEmpty LIC vector; adds a tdTomato gene to the C-terminus of your protein of interest.DepositorTypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-Chrimson-TdTomato
Plasmid#209202PurposeCre-dependent expression of mCherry-tagged Chrimson; OptogeneticsDepositorInsertChrimson
UseAAVTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-fwd[Chrimson-tdT]
Plasmid#59137PurposeExpression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)DepositorInsertChrimson-tdTomato
UseCre/LoxTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only