159,428 results

1. pcDNA3-ApoE3

   Plasmid

   #197147

   Purpose

   Expresses ApoE3 in Mammalian cell

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   ApoE3 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. myc-Raptor

   Plasmid

   #1859

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mPiezo2-CMV-Sport6

   Plasmid

   #81073

   Purpose

   mouse Piezo2

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

   Insert

   Piezo2 (please see depositor comments below) (Piezo2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223-CSNK2A1

   Plasmid

   #23678

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CSNK2A1 (CSNK2A1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. L0-I35 p35S-XVE-pEstradiol GG0-GG1

   Plasmid

   #195904

   Purpose

   Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   p35S-XVE-LexA-p35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP3665

   Plasmid

   #222351

   Purpose

   Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. [pSK122] pETDuet GST-p18(delta5)

   Plasmid

   #136144

   Purpose

   bacterial expression of GST-p18

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   GST-p18
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18T-mini-Tn7T-hph-Ptet2

   Plasmid

   #218368

   Purpose

   Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG:H2B-EGFP

   Plasmid

   #32599

   Depositor

   Virginia Papaioannou, Anna-Katerina Hadjantonakis

   Article

   Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

   Insert

   H2B-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-basP:fLuc-TK:rLuc

    Plasmid

    #138369

    Purpose

    Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p(U6a-BsaI-gRNA)

    Plasmid

    #65955

    Purpose

    Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1hSIRT5_HA

    Plasmid

    #24483

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 5 (SIRT5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LgBiT-ST

    Plasmid

    #207633

    Purpose

    A plasmid encoding LgBiT fused to SpyTag

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    LgBiT-SpyTag
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. [pSK24] pACYCDuet His-c7orf59|HBXIP

    Plasmid

    #136143

    Purpose

    bacterial expression of c7orf59 and HBXIP

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 HA-Sumo2 WT

    Plasmid

    #48967

    Purpose

    Expresses HA SUMO2 Wild Type in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLSB-HYG

    Plasmid

    #166699

    Purpose

    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    adh15 promoter and tRNA promoter

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IL-8 toehold switch sensor

    Plasmid

    #111908

    Purpose

    Toehold switch sensor to detect IL-8 mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    IL8 toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCoofy55

    Plasmid

    #122007

    Purpose

    Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His10-SumoStar (Life Sensors) and Multi2: dependi…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRKH2

    Plasmid

    #71312

    Purpose

    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. All-in-one lenti-BE4-NG

    Plasmid

    #200448

    Purpose

    Lentivirus vector for base editing

    Depositor

    Benjamin Cravatt

    Article

    Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223_TRAF3_WT

    Plasmid

    #81755

    Purpose

    Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTRK892

    Plasmid

    #71803

    Purpose

    Expression of mutated L. gasseri gusA from strong Ppgm promoter

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

    Insert

    gusA3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D573A

    Promoter

    Ppgm

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUS212

    Plasmid

    #84580

    Purpose

    E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His6_MBP_FXR1P

    Plasmid

    #198694

    Purpose

    Expression of human FXR1 protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FXR1P (FXR1 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

    Plasmid

    #164458

    Purpose

    AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. psPax2-D64V-NC-mNeon

    Plasmid

    #196509

    Purpose

    mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    mNeon
    

    Use

    Tags

    mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge