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We narrowed to 13,299 results for: EGFP

Showing: 5071 - 5100 of 13299 results

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-PEmax-P2A-EGFP (LM1589)

Plasmid

#223136

Purpose

pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized PEmax-P2A-EGFP

Use

CRISPR

Tags

BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

Expression

Mammalian

Mutation

M-MLV mutations from PE2 (Anzalone et al. Nature …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-EGFP-WPRE-pA

Plasmid

#37084

Purpose

Cre-dependent expression of EGFP

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-on

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A1-Neo

Plasmid

#189742

Purpose

The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight eGFP Blast (w782-1)

Plasmid

#26584

Purpose

2nd generation lentiviral vector

Depositor

Eric Campeau

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral; Tet-on advanced vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Plasmid

#39761

Depositor

Pamela Raymond

Article

Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

Insert

zebrafish gfap promoter- EGFP (gfap Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

gfap

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-AviTag-ALFAtag-eGFP

Plasmid

#195854

Purpose

Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

6xHis-AviTag-ALFAtag-eGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP Δvpu/env

Plasmid

#101345

Purpose

Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta vpu/Env

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHIV-ODD-EGFP-IRES-dTomato

Plasmid

#87218

Purpose

lentiviral expression of ODD-EGFP

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a ODD domain (HIF1A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

ODD domain

Promoter

EF1

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMVTRE3G eGFP Blast (w818-1)

Plasmid

#27568

Depositor

Eric Campeau

Insert

Enhance Green Fluorescent Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-rat PKA-RIIbeta-mEGFP

Plasmid

#45523

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta tagged by mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

Plasmid

#69100

Purpose

URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

Depositor

David Botstein

Inserts

yZ3EV cassette
Z3 promoter
yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mouse PKA-Calpha-mEGFP

Plasmid

#45521

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA catalytic subunit alpha tagged by mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGal-Trans-Myc-eGFP-KDEL

Plasmid

#177729

Purpose

ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Use

Tags

KDEL, Myc, and eGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

Plasmid

#168998

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Unspecified

Tags

EGFP and mRFP1

Expression

Mutation

Promoter

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-eGFP-WPRE

Plasmid

#218743

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183606

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Mutation

Promoter

Mouse Pgk1

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-WT-Full-length

Plasmid

#194548

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

Plasmid

#119061

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

E1b minimal promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

Plasmid

#181745

Purpose

dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

human/zebrafish codon optimized SpCas9

Use

In vitro transcription; t7 promoter

Tags

NLS(SV40)-3xFLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE-EGFP_IRES-Blast

Plasmid

#213113

Purpose

Expression of MinE-EGFP in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE-EGFP (minE Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII-ShadowG

Plasmid

#167475

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-hygro-EGFP-DFCP1

Plasmid

#174651

Purpose

Vector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

EGFP-DFCP1 (ZFYVE1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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