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We narrowed to 7,509 results for: PAC

Showing: 6761 - 6800 of 7509 results

  1. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159501
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159501-plasmid-map-sequence-id-311777

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  2. pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159502
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159502-plasmid-map-sequence-id-311727

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  3. pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159504
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159504-plasmid-map-sequence-id-311728

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  4. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159506
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159506-plasmid-map-sequence-id-311732

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  5. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159508
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(0)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159508-plasmid-map-sequence-id-311756

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  6. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(16)nts_Tornado-Corn

    Plasmid
    #159511
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 16 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(16)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159511-plasmid-map-sequence-id-311774

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  7. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(19)nts_Tornado-Corn

    Plasmid
    #159514
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 19 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(19)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159514-plasmid-map-sequence-id-311759

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  8. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(20)nts_Tornado-Corn

    Plasmid
    #159515
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 20 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(20)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159515-plasmid-map-sequence-id-311758

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  9. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159499
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159499-plasmid-map-sequence-id-311745

    Enlarge

  10. pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159498
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159498-plasmid-map-sequence-id-311780

    Enlarge

  11. pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn

    Plasmid
    #159496
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4) _HairpinDistance(0)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159496-plasmid-map-sequence-id-311762

    Enlarge

  12. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159495
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159495-plasmid-map-sequence-id-311767

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  13. SMT215

    Plasmid
    #134818
    Purpose
    SMT205 with DHFR-FLAG-tag, western blot
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    134818-plasmid-map-sequence-id-313944

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  15. pYPQ265H

    Plasmid
    #149554
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    hAID-ziSpyMacCas9(D10A)-UGI
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149554-plasmid-map-sequence-id-308763

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  17. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

    Enlarge

  18. pYPQ266B

    Plasmid
    #149555
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    ziSpyMacCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Promoter
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149555-plasmid-map-sequence-id-306140

    Enlarge

  19. pMM0648

    Plasmid
    #129009
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129009-plasmid-map-sequence-id-259442

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  20. pC0.007

    Plasmid
    #119671
    Purpose
    Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.
    Depositor
    Alistair McCormick
    Article
    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do
    Insert
    Pcpc560-Dx2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    bp 89 T to C, bp 187 C to A, Duplicated downstrea…
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119671-plasmid-map-sequence-id-237673

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  30. pMBEC6

    Plasmid
    #187598
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187598-plasmid-map-sequence-id-367542

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  31. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

    Enlarge

  32. pDGM6

    Plasmid
    #110660
    Purpose
    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.
    Depositor
    David Russell
    Article
    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.
    Insert
    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110660-plasmid-map-sequence-id-337382

    Enlarge

  34. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

    Enlarge

  35. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

    Enlarge

  36. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

    Enlarge

  38. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160730-plasmid-map-sequence-id-357931

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Showing: 6761 - 6800 of 7509 results