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20,120 results

Showing: 5101 - 5130 of 20120 results

  1. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  14. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Marcella Patrick
    Has Service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available Since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105921-plasmid-map-sequence-id-443051

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  16. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Has Service
    Cloning Grade DNA
    Insert
    BirA
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available Since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    35700-plasmid-map-sequence-id-228311

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  19. pLJM1-EGFP-GRAM-W

    Plasmid
    #211704
    Purpose
    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211704-plasmid-map-sequence-id-419216

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  21. pcDNA3-TLR4-YFP

    Plasmid
    #13018
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 4 (TLR4 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13018-plasmid-map-sequence-id-339915

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  29. pcDNA3-TLR9-YFP

    Plasmid
    #13642
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 9 (TLR9 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13642-plasmid-map-sequence-id-392328

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Showing: 5101 - 5130 of 20120 results