Addgene

We narrowed to 10,228 results for: T7

Showing: 6841 - 6880 of 10228 results

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pcDNA6-rbN1 S77D

Plasmid

#32947

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

slc9a3r1 (SLC9A3R1 rabbit)

Use

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Serine 77 mutated to Aspartic Acid

Promoter

CMV

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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Cwc25-pRSETA

Plasmid

#61043

Purpose

Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Cwc25

Use

Tags

TEV site, Histidine Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
Ubiquitin (1-76; L71Y)

Plasmid

#110756

Purpose

Bacterial expression of human Ubiquitin (1-76; L71Y)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Use

Tags

Expression

Bacterial

Mutation

L71Y

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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HOXD13 (chicken) HIS-tag pET

Plasmid

#8555

Depositor

Corey Largman

Article

Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

Insert

HOXD13 (HOXD13 )

Use

Tags

His and T7

Expression

Bacterial

Mutation

contains 10 nt 5' to ATG start; makes prote…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
TAL3420

Plasmid

#47139

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-def6a-left (def6a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3219

Plasmid

#42682

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg5-right (atg5 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
TAL3217

Plasmid

#42680

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg12-right (atg12 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
TAL3218

Plasmid

#42681

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg5-left (atg5 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3216

Plasmid

#42679

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg12-left (atg12 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pKV006

Plasmid

#64764

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFvL106

Plasmid

#64751

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, URA selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 ATF3

Plasmid

#67057

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ATF3 (ATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

M2I

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
TAL3315

Plasmid

#42778

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-meis2a-right (meis2.2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3314

Plasmid

#42777

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-meis2a-left (meis2.2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL3

Plasmid

#67418

Purpose

Expression of hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3035

Plasmid

#41209

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-bsx-Right (bsx Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only
TAL3043

Plasmid

#41217

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-cebpd-Right (cebpd Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3042

Plasmid

#41216

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-cebpd-Left (cebpd Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
gabra5_R (TAL3015)

Plasmid

#36007

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
gabra5_L (TAL3014)

Plasmid

#36006

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3034

Plasmid

#41208

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-bsx-Left (bsx Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 I136A, I139A, M142A

Plasmid

#74617

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGB1-mCRT(P-domain)

Plasmid

#83519

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Use

Tags

GB1 and His

Expression

Bacterial

Mutation

P domain (contains residues 187-283)

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pGB1-mCRT(C-domain)

Plasmid

#83518

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Use

Tags

GB1 and His

Expression

Bacterial

Mutation

C domain (contains residues 340-399)

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
TAL3439

Plasmid

#47158

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-fastkd3-right (im:7147223 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pET16b-Trx80

Plasmid

#36945

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Trx80 (truncated Trx) (TXN Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

premature stop inserted after K80 in Trx1 gene

Promoter

T7

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-ybbR-HIS-10FNIII(x4)-DocI

Plasmid

#58712

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

10FNIII(x4)-DocI

Use

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Human Mevalonate diphosphate decarboxylase (MDD)

Plasmid

#52431

Purpose

bacterial expression of human Mevalonate diphosphate decarboxylase (MDD)

Depositor

Henry Miziorko

Article

Voynova et al Arch Biochem Biophys. 2008 Dec 1;480(1):

Insert

mevalonate diphosphate decarboxylase (MVD Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
TAL3449

Plasmid

#47168

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-hcrt-right (hcrt Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3448

Plasmid

#47167

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-hcrt-left (hcrt Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3430

Plasmid

#47149

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-eomesb-left (eomesb Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3275

Plasmid

#42738

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-hemogen-site1-right (hemgn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3277

Plasmid

#42740

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-hemogen-site2-right (hemgn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3276

Plasmid

#42739

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-hemogen-site2-left (hemgn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
TAL3274

Plasmid

#42737

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-hemogen-site1-left (hemgn Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3535

Plasmid

#47254

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sdcbp-right (sdcbp Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3534

Plasmid

#47253

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sdcbp-left (sdcbp Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3421

Plasmid

#47140

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-def6a-right (def6a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Showing: 6841 - 6880 of 10228 results

Results per page:

Sort results by: