We narrowed to 10,761 results for: T7
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Plasmid#112628PurposeEncodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.DepositorInsertrTetR-NS3-VP64-p65
TagsAU1 (N term on NS3), HA (C term on NS3), and T7 (…ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-3xFlag-IFIT5
Plasmid#53561Purposebacterial expression of IFIT5DepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABECD
Plasmid#66536PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoterDepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyExpressionBacterialPromoterMutant T7 Promoter - G6Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-Uricase
Plasmid#182401PurposeBacterial expression of Vesicle Nucleating peptide-uricase fusionDepositorInsertVNp-uricase
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOtop3_pGEMHE
Plasmid#114676PurposeThis construct was used to express mouse Otop3 in Xenopus laevis oocytesDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVT/Smarca4
Plasmid#122146PurposeFor making Smarca4 RNADepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
TagsHisExpressionBacterialPromoterT7Available SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Histone H2B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45216DepositorInsertHistone H2B-targeted Aurora B FRET sensor
ExpressionMammalianPromoterT7Available SinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
TagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNG1256
Plasmid#149710PurposeExpresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complexDepositorAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1363
Plasmid#166861PurposeExpression of codon optimized SARS-COV-2 Nsp8 in E. coliDepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cut-P(11)4
Plasmid#185785PurposeEncodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertCut-P(11)4
TagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorInsertMMLV-RT
TagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB17
Plasmid#52527Purposeserves as template for PCR-mediated fusion of U6-promoter with sgRNADepositorInsertDrosophila U6C promoter
UseCRISPR; Blunt cloning kitTagsT7-promoterExpressionInsectAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-trAtCCD7-OsD27(pYL726 )
Plasmid#178285PurposeCo-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from ArabidopsisDepositorInsertsOsD27
trAtCCD7
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIA1362
Plasmid#166860PurposeExpression of codon optimized SARS-COV-2 Nsp7 in E. coliDepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-de4GFP
Plasmid#85472PurposeInducible expression of dual emitting pH sensitive GFP in bacterial cellsDepositorInsertde4GFP
Tags6xHisExpressionBacterialMutationS65T/C48S/H148C/T203CPromoterT7Available SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
Tags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT tetramutant for Split-PE (pEH101)
Plasmid#190109PurposeExpresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)DepositorInsertMarathon-RT
TagsbpNLSExpressionMammalianMutationD14R, D74R, N116K, N197RPromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pGEMHE
Plasmid#114675PurposeThis construct was used to express mouse Otop2 in Xenopus laevis oocytesDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7 promoterAvailable SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
Tags10xHis-MBPExpressionBacterialMutationresidues 56–714; See paper for descriptionPromoterT7Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Caspase-6
Plasmid#100102PurposeBacterial expression of Caspase-6DepositorAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEThT-CAHS1
Plasmid#90027PurposeTo express His-tagged CAHS1 in bacteriaDepositorInsertCAHS1
TagsHisExpressionBacterialPromoterT7Available SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P6
Plasmid#122649PurposeExpresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-HIS-CBM-Xmod-DocIII
Plasmid#91698PurposeCBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGGDepositorInsertGGG-HIS-CBM-Xmod-DocIII
TagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEW3: pET-32a Dot1L(2-416)
Plasmid#124098PurposeDot1L(2-416) expression plasmidDepositorAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
Tags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-LPETGG
Plasmid#91697PurposeCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGGDepositorInsertCohIII-CBM-HIS-LPETGG
TagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDM4-GLY
Plasmid#49808PurposeEncodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.DepositorInsertsphosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase
ExpressionBacterialPromoterT7Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_switch
Plasmid#132722PurposeT7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N20_switch
ExpressionBacterialAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_trigger
Plasmid#132726PurposeT7 RNAP-driven expression of 3WJ repressor index 20 trigger RNADepositorInsert3WJ_N20_trigger
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only