We narrowed to 10,458 results for: T7

1. pmCellFree_KA1_ZIKV NS3

   Plasmid

   #145345

   Purpose

   Cell-free/ mammalian expression vector for Zika virus NS3 protein with N-term eGFP

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS3
   

   Use

   Tags

   8xHis and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   April 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmCellFree_KA1_ZIKV NS4A

   Plasmid

   #145346

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFP

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS4A
   

   Use

   Tags

   8xHis and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   April 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-sfGFP-SpyTag003 Loop A

   Plasmid

   #171776

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues V…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-PKAsub-YPet-NES

   Plasmid

   #138203

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HpMscS

   Plasmid

   #78310

   Purpose

   H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

   Depositor

   Douglas Rees

   Article

   Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

   Insert

   Mechanosensitive channel of small conductance
   

   Use

   Tags

   6x His Tag and FLAG Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCellFree_G03 NFKB1

   Plasmid

   #67126

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Gcm2TEASY

   Plasmid

   #41031

   Depositor

   Nancy Manley

   Article

   Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

   Insert

   Gcm2 in situ probe (Gcm2 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   contains nucleotides 584-1510 of GenBank accessio…

   Promoter

   T7 sense; SP6 antisense

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-JNKsubDD-YPet-NES

   Plasmid

   #84633

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsubDD-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CyPet-FKBP-E2

   Plasmid

   #175244

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BB-­NIC-­II-­HisN-­TET12

    Plasmid

    #45496

    Depositor

    Roman Jerala

    Article

    Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

    Insert

    TET12
    

    Use

    Synthetic Biology

    Tags

    6xHistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b-At 14-3-3 epsilon

    Plasmid

    #44936

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 epsilon (GRF10 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221-kcnj11

    Plasmid

    #173139

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj11 (kcnj11 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM6-Vv4CL-PhCHS-CmCHI

    Plasmid

    #73401

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Vv4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-BCA-P(11)4

    Plasmid

    #185780

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-P(11)4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM6-Pc4CL-PhCHS-MsCHI

    Plasmid

    #73396

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    PhCHS
    MsCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSFDuet-1_VNp-DARPinOFF7

    Plasmid

    #182397

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MEIS1A-pET

    Plasmid

    #21015

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

    Insert

    MEIS1A (Meis1 Mouse)
    

    Use

    Tags

    HIS and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G03 DDX5

    Plasmid

    #67111

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pSLS.540

    Plasmid

    #184959

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    fabH donor G954A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETM33_OXSR1_CCT

    Plasmid

    #178482

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    OXSR1_OXSR1 (OXSR1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TAL2295

    Plasmid

    #36728

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    APC-TALEN_Right (APC Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pET21a - SodB

    Plasmid

    #89613

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase B
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

    Plasmid

    #185711

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed I770 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLyGo-Ec-6

    Plasmid

    #163132

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYZ_3WJrep_N11_trigger

    Plasmid

    #132733

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 11 trigger RNA

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N11_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. B. fragilis mRNA toehold switch sensor

    Plasmid

    #110706

    Purpose

    Toehold switch sensor to detect a species specific mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis species specific toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-PLS-KP2_rpoA-U

    Plasmid

    #190956

    Purpose

    Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-PLS-KP6-A66S_rpoA-U

    Plasmid

    #190969

    Purpose

    Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-PLS-KP3_rpoA-U

    Plasmid

    #190960

    Purpose

    Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-PLS-KP6-E67A_rpoA-U

    Plasmid

    #190973

    Purpose

    Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSET-triple mutation-pa-GCaMP6f

    Plasmid

    #67570

    Purpose

    bacterial expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    pa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    V115H, L64F, T65S

    Promoter

    T7

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. hisSUMO-Gli1

    Plasmid

    #87225

    Purpose

    Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    GLI1 (Gli1 Mouse)
    

    Use

    Tags

    hisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p158

    Plasmid

    #62617

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSETb-rsGreenF

    Plasmid

    #78181

    Purpose

    Expresses rsGreenF in E. coli strains.

    Depositor

    Peter Dedecker

    Article

    Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

    Insert

    rsGreenF
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Hox A13b

    Plasmid

    #36468

    Depositor

    Robert Ho

    Article

    Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

    Insert

    hoxA13b (hoxa11b Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    F232C

    Promoter

    T7

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pDEST17-6xHis-ARL2

    Plasmid

    #67474

    Purpose

    Expression of hArl2 with N-term His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL2 (ARL2 Human)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pCellFree_G03 FYN

    Plasmid

    #67106

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FYN (FYN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pmCellFree_KA2_ZIKV NS2A

    Plasmid

    #145363

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2A
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBluescript-mouse eIF2C4

    Plasmid

    #50356

    Purpose

    Mouse eIF2C4 cDNA clone

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C4 (Ago4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge