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Showing: 8551 - 8600 of 10312 results

  1. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19

    Plasmid
    #111733
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q19 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111733-plasmid-map-sequence-id-304484

    Enlarge

  3. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q15

    Plasmid
    #111732
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q15 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111732-plasmid-map-sequence-id-220471

    Enlarge

  4. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q78

    Plasmid
    #111737
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q78 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111737-plasmid-map-sequence-id-226923

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  5. SARS CoV-2 HexaPro spike (bio-His)

    Plasmid
    #166856
    Purpose
    Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.
    Depositor
    Evelien Bunnik
    Article
    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d
    Insert
    SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
    Tags
    8xHis and Biotinylation site
    Expression
    Mammalian
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166856-plasmid-map-sequence-id-327668

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  6. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q66

    Plasmid
    #111735
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q66 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111735-plasmid-map-sequence-id-226804

    Enlarge

  7. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139

    Plasmid
    #111738
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q139 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111738-plasmid-map-sequence-id-247719

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  8. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48

    Plasmid
    #111747
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q48 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111747-plasmid-map-sequence-id-220485

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  9. pcDNA3-sACE2v2.4(732)-8h

    Plasmid
    #154103
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-G…
    Promoter
    CMV
    Available Since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154103-plasmid-map-sequence-id-301646

    Enlarge

  10. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51

    Plasmid
    #111734
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q51 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111734-plasmid-map-sequence-id-220474

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  11. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73

    Plasmid
    #111728
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q73 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111728-plasmid-map-sequence-id-226784

    Enlarge

  12. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73

    Plasmid
    #111724
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q73 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111724-plasmid-map-sequence-id-226887

    Enlarge

  13. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79

    Plasmid
    #111725
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q79 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111725-plasmid-map-sequence-id-226810

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  14. pαH-S-GSAS-B.1.1.7

    Plasmid
    #171747
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171747-plasmid-map-sequence-id-337765

    Enlarge

  15. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available Since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154105-plasmid-map-sequence-id-304581

    Enlarge

  16. pcDNA3-sACE2v2(732)-8h

    Plasmid
    #154102
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154102-plasmid-map-sequence-id-301648

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  17. pαH-S-GSAS-P1-like

    Plasmid
    #173789
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    173789-plasmid-map-sequence-id-340758

    Enlarge

  18. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52

    Plasmid
    #111748
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q52 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111748-plasmid-map-sequence-id-220486

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  20. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24

    Plasmid
    #111742
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q24 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111742-plasmid-map-sequence-id-220476

    Enlarge

  22. pαH-S-GSAS-B.1.351.v2

    Plasmid
    #173786
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    173786-plasmid-map-sequence-id-340773

    Enlarge

  23. pαH-S-GSAS-B.1.351.v6

    Plasmid
    #173791
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 6
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    173791-plasmid-map-sequence-id-339984

    Enlarge

  24. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25

    Plasmid
    #111743
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q25 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111743-plasmid-map-sequence-id-220479

    Enlarge

  26. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

    Enlarge

  27. pαH-S-GSAS-B.1.351

    Plasmid
    #171749
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171749-plasmid-map-sequence-id-337764

    Enlarge

  28. pαH-S-GSAS-D614G-K417N-E484K-N501Y

    Plasmid
    #171750
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501Y
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171750-plasmid-map-sequence-id-337761

    Enlarge

  29. pαH-S-GSAS-D614G-deltaFV

    Plasmid
    #171743
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171743-plasmid-map-sequence-id-337714

    Enlarge

  30. pHis-TEV-Nsp15-E266Q

    Plasmid
    #218803
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    E266Q (G to C)
    Available Since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218803-plasmid-map-sequence-id-433302

    Enlarge

  31. pHis-TEV-Nsp15-V66L

    Plasmid
    #219524
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    V66L (G to C)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219524-plasmid-map-sequence-id-433377

    Enlarge

  32. pHis-TEV-Nsp15-A81V

    Plasmid
    #219525
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    A81V (C to T)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219525-plasmid-map-sequence-id-433375

    Enlarge

  33. pHis-TEV-Nsp15-D183N

    Plasmid
    #219526
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    D183N (G to A)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219526-plasmid-map-sequence-id-433305

    Enlarge

  34. pHis-TEV-Nsp15-H234A

    Plasmid
    #219529
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    H234A (CAC to GCG)
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219529-plasmid-map-sequence-id-433314

    Enlarge

  37. pT2iApowriter2(#336)

    Plasmid
    #184104
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184104-plasmid-map-sequence-id-364017

    Enlarge

  38. pT22Apowriter2(#340)

    Plasmid
    #184105
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184105-plasmid-map-sequence-id-363997

    Enlarge

  39. pT22iuasvApoWtEos3N(#486)

    Plasmid
    #184107
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184107-plasmid-map-sequence-id-363989

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  40. pT22iuasvApoWChe(#488)

    Plasmid
    #184108
    Purpose
    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184108-plasmid-map-sequence-id-363970

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  41. pαH-S-GSAS-B.1.351.v4

    Plasmid
    #173788
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    173788-plasmid-map-sequence-id-352110

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  48. Spike Display_Part 1-5 DO

    Plasmid
    #172726
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172726-plasmid-map-sequence-id-335212

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  49. pcDNA3-sPDGFRalpha-Fc-Y206S

    Plasmid
    #136439
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524) …
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136439-plasmid-map-sequence-id-271696

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  50. pcDNA3-sPDGFRalpha-Fc-V242K

    Plasmid
    #136440
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524) …
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136440-plasmid-map-sequence-id-271678

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