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Showing: 6921 - 6960 of 167150 results

  4. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

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  8. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

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  24. CRISPRoff-mScarletI

    Plasmid
    #217783
    Purpose
    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217783-plasmid-map-sequence-id-468886

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  32. pmScarlet3H-C1

    Plasmid
    #242975
    Purpose
    C1 Cloning vector for mammalian cells incorporating mScarlet3 -H red fluorescent protein
    Depositor
    Dorus Gadella
    Insert
    mScarlet3-H
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242975-plasmid-map-sequence-id-492373

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  33. snuABE4.2

    Plasmid
    #246014
    Purpose
    snuABE4.1 with MLH1-SB fused to the C-terminus via P2A.
    Depositor
    Sangsu Bae
    Article
    Im et al bioRxiv 2025.07.19.664718
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
    Tags
    MLH1-SB
    Expression
    Mammalian
    Mutation
    E438Q, I536S, E637F, with 10 amino acids truncati…
    Promoter
    CMV
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246014-plasmid-map-sequence-id-492308

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  34. snuABE4.1

    Plasmid
    #246013
    Purpose
    snuABE4.1 composed of PhADAR(E438Q, I536S, E637F) with 10a.a truncation fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
    Depositor
    Sangsu Bae
    Article
    Im et al bioRxiv 2025.07.19.664718
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)
    Expression
    Mammalian
    Mutation
    E438Q, I536S, E637F, with 10 amino acids truncati…
    Promoter
    CMV
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246013-plasmid-map-sequence-id-492306

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  35. snuABE3.1

    Plasmid
    #246011
    Purpose
    snuABE3.1 composed of PhADAR(E438Q) fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.
    Depositor
    Sangsu Bae
    Article
    Im et al bioRxiv 2025.07.19.664718
    Insert
    Pediculus humanus ADAR deaminase domain (E438Q mutant)
    Expression
    Mammalian
    Mutation
    E438Q
    Promoter
    CMV
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246011-plasmid-map-sequence-id-492301

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  36. pAG mIgG2a Anti-c-Myc [9E10]

    Plasmid
    #242955
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain constant region; to be used with pAG mKappa Anti-c-Myc light chain [9E10] (Plasmid 242956) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain
    Tags
    Mouse IgG2a
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242955-plasmid-map-sequence-id-484469

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  37. pAG mKappa Anti-c-Myc [9E10]

    Plasmid
    #242956
    Purpose
    Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse kappa light chain constant region; to be used with pAG mIgG2a Anti-c-Myc [9E10] heavy chain (Plasmid 242955) to make the antibody.
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242956-plasmid-map-sequence-id-485246

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Showing: 6921 - 6960 of 167150 results