We narrowed to 78,760 results for: TRI

1. pRC/CMV-JAK1-VSV

   Plasmid

   #139356

   Purpose

   Expression of JAK1 protein

   Depositor

   Sandra Pellegrini

   Article

   Richter et al J Biol Chem. 1998 Sep 18;273(38):24723-9

   Insert

   JAK1 cDNA with 300nt of 5'UTR (JAK1 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLM-mCherry-Klf4

   Plasmid

   #23243

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNH605-HSE-YFP

   Plasmid

   #172131

   Purpose

   Fluorescent reporter for Hsf1 activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xHSE-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of the H…

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1 TMEM192-Flag

   Plasmid

   #112742

   Purpose

   express pLJM1 TMEM192-Flag

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   TMEM192-Flag (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO-FUW-eGFP-RHOA-T19N

   Plasmid

   #73082

   Purpose

   Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-T19N

   Depositor

   Andrew Putnam

   Article

   Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

   Insert

   RHOA-T19N (RHOA Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

   Plasmid

   #158953

   Purpose

   Express human RAD51(T309A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed threonine 309 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

   Plasmid

   #130991

   Purpose

   AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet-Kv2.1

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM-DCA-muGFP

   Plasmid

   #166727

   Purpose

   AAV, encodes monomeric ultrastable GFP (muGFP)

   Depositor

   Daniel Scott

   Article

   Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

   Insert

   muGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Chicken Beta Actin

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWPI-UPP1-3xFLAG

   Plasmid

   #201644

   Purpose

   Over-expression of 3xFLAG-tagged human UPP1

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLM-vexGFP-Oct4

    Plasmid

    #22240

    Purpose

    2nd generation lentiviral vector

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    vexGFP_2A_OCT4 (POU5F1 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

    Plasmid

    #158952

    Purpose

    Express human RAD51(F129A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed phenylalanine 129 to alanine

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-6P-1-INTS3-FL

    Plasmid

    #128415

    Purpose

    Express INTS3 full-length in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    full-length

    Promoter

    tac promoter

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3xFLAG-CMV10-hAtg13

    Plasmid

    #22872

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    autophagy-related genes 13 (ATG13 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

14. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-ChRmine-eYFP-WPRE

    Plasmid

    #130992

    Purpose

    AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. single polypeptide FPX biosensor for caspase-1

    Plasmid

    #60885

    Purpose

    FPX biosensor for caspase-1

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-1
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. xlox dNGFR-TERT

    Plasmid

    #69805

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

    Depositor

    David Ott

    Article

    Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

    Insert

    hTERT reverse transcriptase (TERT Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    MLV promoter

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. ScSpe1 pET-20b(+)

    Plasmid

    #117145

    Purpose

    Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    SPE1
    

    Use

    Tags

    Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG27

    Plasmid

    #226742

    Purpose

    Expresses a reporter for a mitochondrial matrix protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    COX4 (COX4I1 Human)
    

    Use

    Lentiviral

    Tags

    GFP1-10

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

    Plasmid

    #74947

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Oct3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBa-FRB-3myc-Rab7

    Plasmid

    #64210

    Purpose

    Rab7 with N-terminal FRB and triple myc tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Rab7 (Rab7 Mouse)
    

    Use

    Tags

    FRB and triple myc tag (3myc)

    Expression

    Mammalian

    Mutation

    Promoter

    chicken Beta Actin

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hCEP120-Myc

    Plasmid

    #50381

    Purpose

    Tet-On inducible expression of Myc-tagged hCEP120

    Depositor

    Tang Tang

    Article

    Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

    Insert

    Human CEP120 (CEP120 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    This hCEP120 contains 4 SNPs (rs6876883, rs659544…

    Promoter

    CMV-2xTetO

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFBP_2xNbLYS1::LUZ

    Plasmid

    #189918

    Purpose

    Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.

    Depositor

    Adam Steinbrenner

    Article

    Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Various

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNH605-PACE-YFP

    Plasmid

    #172002

    Purpose

    Fluorescent reporter for Rpn4 activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xPACE-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1 core promotor with 4 tandem repeats of Rpn4 …

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LENTICRISPR-TKT_sgRNA2

    Plasmid

    #201625

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LENTICRISPR-TKT_sgRNA1

    Plasmid

    #201624

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-C3-PLK4 K41M-3xFLAG

    Plasmid

    #69838

    Purpose

    This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    K41M

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSI2-DCD

    Plasmid

    #166968

    Purpose

    Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-DCD
    

    Use

    Retroviral

    Tags

    MSI2-DCD

    Expression

    Mammalian

    Mutation

    N495S

    Promoter

    MSCV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCEP4-myc-HLA-B57

    Plasmid

    #135516

    Purpose

    Mammalian expression of myc-tagged HLA-B*57:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*57:1 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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