We narrowed to 17,840 results for: Mut

1. pCEP4-myc-CCR5-VC

   Plasmid

   #98966

   Purpose

   myc-tagged human CCR5 fused to C-terminus of split Venus

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   Signal/leader sequence from Influenza A virus HA …

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShuttle-EGFP-CLASP2 340-1362 9xS/A

   Plasmid

   #24373

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1362) 9xS/A (CLASP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-Cas9-VQR-GFP_activity_reporter

   Plasmid

   #87156

   Purpose

   This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   GFP and sgRNA targeting GFP (NGA restricted)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-DDR1__PAK1

   Plasmid

   #116813

   Purpose

   Lentiviral expression of DDR1__PAK1 fusion

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   DDR1__PAK1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-FLAG-eSpCas9-plus

   Plasmid

   #126774

   Purpose

   Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCMV:ECFP(loxP)(FRT)HNF1B

   Plasmid

   #31439

   Depositor

   Gerhart Ryffel

   Article

   Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSN672

   Plasmid

   #177362

   Purpose

   Expresses human KIF1A(1-393) fused with leucine zipper and His tag

   Depositor

   Shinsuke Niwa

   Article

   Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

   Insert

   KIF1A (KIF1A Human)
   

   Use

   Tags

   His tag and Leucine zipper

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

   Plasmid

   #184406

   Purpose

   yeast surface display of the SARS-CoV-2 Beta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   K417N+E484K+N501Y

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8/GW/TOPO-ELK1

   Plasmid

   #98616

   Purpose

   Gateway entry TOPO TA cloning vector for ELK1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Q230R

   Promoter

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneo-AR-S81E

    Plasmid

    #171224

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Glu (AR-S81E)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser81Glu mutant (AR Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser81Glu (AR-S81E)

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-ATP2B4__OPTC

    Plasmid

    #116808

    Purpose

    Lentiviral expression of ATP2B4__OPTC fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ATP2B4__OPTC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-hDDB2-T338M-V5

    Plasmid

    #167470

    Purpose

    ectopic expression of hDDB2 in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

    Insert

    DDB2 (DDB2 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    T338M

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-ERGIC1__RANBP17

    Plasmid

    #116816

    Purpose

    Lentiviral expression of ERGIC1__RANBP17 fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERGIC1__RANBP17
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-hDDB2-K244E-V5

    Plasmid

    #167471

    Purpose

    ectopic expression of hDDB2 in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

    Insert

    DDB2 (DDB2 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    K244E

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Tag4A-MMP8 (P78S)

    Plasmid

    #29546

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    P78S

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pLV-PLXNB2-mRBD

    Plasmid

    #86240

    Purpose

    Lentivirus for expression of human Plexin-B2 with mutant RBD domain

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    PLXNB2 with point mutations of RBD domain

    Promoter

    hPGK

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

    Plasmid

    #154779

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    LaG17-SynNotch
    

    Use

    Tags

    LaG17

    Expression

    Mammalian

    Mutation

    204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-MCM5__LYN

    Plasmid

    #116828

    Purpose

    Lentiviral expression of MCM5__LYN fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MCM5__LYN
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myc-Idax DBM

    Plasmid

    #68554

    Purpose

    This plasmid expresses full length IDAX containing a DNA binding mutation.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    IDAX-DBM (Cxxc4 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T162A, H164A and Q165A mutations

    Promoter

    EF1a

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-EGFP-Cx43ML-N1

    Plasmid

    #49860

    Purpose

    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    All internal Methionines mutated to Leucine. No s…

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

    Plasmid

    #51254

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Asp

    Promoter

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

22. H14-MBP-SUMO-Cys-Linker-BAF_L58R

    Plasmid

    #101780

    Purpose

    Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Tags

    His14-MBP-SUMO

    Expression

    Bacterial

    Mutation

    L58R mutation

    Promoter

    T5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-WNK1__PRMT8

    Plasmid

    #116845

    Purpose

    Lentiviral expression of WNK1__PRMT8 fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    WNK1__PRMT8
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

    Plasmid

    #119312

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-500

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)

    Plasmid

    #119311

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-376

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

    Plasmid

    #119316

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 481-852

    Promoter

    CMV

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAdEasy-EGFP-CLASP2 340-1084 9xS/A

    Plasmid

    #24525

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1084) 9xS/A (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant. M…

    Promoter

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

28. B-eSpCas9

    Plasmid

    #126761

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTE3320

    Plasmid

    #107529

    Purpose

    Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hLbCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

    Plasmid

    #68346

    Purpose

    Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

    Inserts

    3xgRNA;PTENA, p53B,SMAD4A
    FlPO
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. B-evoSpCas9

    Plasmid

    #126765

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3.1Xpress-BcrGAP R1090A

    Plasmid

    #38176

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
    

    Use

    Tags

    Xpress

    Expression

    Mammalian

    Mutation

    aa 1004-1271 with R1090A point mutation

    Promoter

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pTAMAHISTEV_PfeAR480A-Q482A

    Plasmid

    #128946

    Purpose

    expresses R480A Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHAGE-CDC42BPB__RGS6

    Plasmid

    #116810

    Purpose

    Lentiviral expression of CDC42BPB__RGS6 fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDC42BPB__RGS6
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHAGE-CDC42BPB__SYNJ2BP

    Plasmid

    #116811

    Purpose

    Lentiviral expression of CDC42BPB__SYNJ2BP fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDC42BPB__SYNJ2BP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pShuttle-EGFP-CLASP2 340-1084 9xS/A

    Plasmid

    #24384

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1084) 9xS/A (CLASP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant. M…

    Promoter

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hnRNPA2_LC_P298L_S285C

    Plasmid

    #104469

    Purpose

    express His tagged P298L S285C hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P298L S285C

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHAGE-TAOK1__PIPOX

    Plasmid

    #116839

    Purpose

    Lentiviral expression of TAOK1__PIPOX fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    TAOK1__PIPOX
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SLC30A10-delta105-107

    Plasmid

    #65205

    Purpose

    Constitutive expression of SLC30A10 delta 105-107 in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Leyva-Illades et al J Neurosci. 2014 Oct 15;34(42):14079-95.

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta 105-107 amino acids

    Promoter

    CMV

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

40. CRY-GalVP16l (B694)

    Plasmid

    #92030

    Purpose

    expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    GalBD-VP16

    Expression

    Mammalian

    Mutation

    NLS sequence of CRY2 mutated

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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