We narrowed to 18,889 results for: Tec

1. pASKt15C+SrtA

   Plasmid

   #64983

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   tetracycline promoter/operator

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 SP2

   Plasmid

   #67109

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SP2 (SP2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. TALEN1258

   Plasmid

   #32281

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting gria3a (gria3a Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and FOKI WT

   Expression

   Mammalian

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDM_noro_S2_lacZA

   Plasmid

   #118816

   Purpose

   T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch S2
   

   Expression

   Bacterial

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. BCJJ344

   Plasmid

   #117503

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtUBI10

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GWF135

   Plasmid

   #133614

   Purpose

   ANR-ANR-BFP-CAAX

   Depositor

   Dustin Maly

   Article

   Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

   Insert

   ANR-ANR-BFP-CAAX
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdEos-Talin-18

   Plasmid

   #57672

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Talin
   

   Tags

   tdEos

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pATD

   Plasmid

   #141012

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   HSV-TK
   

   Expression

   Mammalian

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTMS1

   Plasmid

   #87799

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cx55.5:rasGFP

    Plasmid

    #105939

    Purpose

    in vitro transcription

    Depositor

    Caren Norden

    Article

    Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

    Insert

    Ras
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 USF2

    Plasmid

    #67069

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    USF2 (USF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-PRLSS-SH2-KDEL

    Plasmid

    #209716

    Purpose

    Mammalian expression of SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-SH2-KDEL
    

    Tags

    PRLSS

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. YSBE 596 : pNAT_mCherry

    Plasmid

    #92088

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, NAt selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    mCherry
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSGV Rh Acceptor

    Plasmid

    #64271

    Purpose

    Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB1751

    Plasmid

    #174013

    Purpose

    pDUAL plasmid expressing GFP from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    GFP
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    41nmt1 and 41nmt1 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-zeoCV

    Plasmid

    #73890

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    zeoCV
    

    Expression

    Bacterial, Mammalian, and Y…

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLpC-L-IFP-F[1] (mammalian)

    Plasmid

    #52901

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLpC-L-IFP-F[2] (mammalian)

    Plasmid

    #52902

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 VPS29

    Plasmid

    #67052

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only