164,577 results

1. pLVX-opto-mCasp8

   Plasmid

   #208782

   Purpose

   Inducibly expresses mouse caspase8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mCaspase8 Cry2 (Casp8 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ190

   Plasmid

   #162667

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRG2.1_Puro

   Plasmid

   #125594

   Purpose

   Lentiviral expression of sgRNA with GFP and puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. v5 ABE-eVLP

   Plasmid

   #228467

   Purpose

   Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

   Depositor

   David Liu

   Article

   Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

   Insert

   MMLVgag(C507V)–ABE8e
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   MMLVgag(C507V)

   Promoter

   CMV

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7

   Plasmid

   #11795

   Purpose

   3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

   Depositor

   Luk Parijs

   Article

   Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKNOCK-Cm

   Plasmid

   #46261

   Purpose

   Bacterial targeting

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

   Type

   Empty backbone

   Use

   Bacterial targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 14, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pKLV2-EF1a-BsdCas9-W

   Plasmid

   #67978

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

   Plasmid

   #193294

   Purpose

   episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TUBG1_pLX307

   Plasmid

   #98376

   Purpose

   Lentiviral expression of TUBG1

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TUBG1 (TUBG1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-rtTA

    Plasmid

    #163601

    Purpose

    Expresses rtTA in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    rtTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPR v2-sgLKB1 clone1

    Plasmid

    #162125

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HA-UBA6

    Plasmid

    #136995

    Purpose

    Expresses human UBA6 in mammalian cells

    Depositor

    Marcus Groettrup

    Article

    Pelzer et al J Biol Chem. 2007 Aug 10;282(32):23010-4

    Insert

    UBA6 (UBA6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKL2315

    Plasmid

    #187876

    Purpose

    Expresses Cre recombinase.

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLminP_Luc2P_RE22

    Plasmid

    #90364

    Purpose

    STAT6 - Stat6 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT6

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. DUSP6

    Plasmid

    #27975

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. DHFR-SpCas9-DHFR

    Plasmid

    #85447

    Purpose

    Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-SpCas9-DHFR
    

    Use

    AAV

    Tags

    Destabilized domain (C-terminus version) of E.col…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_NOTCH2_WT

    Plasmid

    #82292

    Purpose

    Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NOTCH2 (NOTCH2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    C946Y

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234441

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Has Service

    AAV1

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

    Plasmid

    #62897

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMVTNT-T7-KPNA2

    Plasmid

    #26678

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 2 (KPNA2 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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