We narrowed to 78,760 results for: TRI

1. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

   Plasmid

   #61582

   Purpose

   Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   Cezanne2 (OTUD7A Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Isoform 2. Deleted aa 463-933.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221 UBR5 ∆PABC

   Plasmid

   #81064

   Purpose

   Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScalps_Puro_mCebpe

   Plasmid

   #79076

   Purpose

   Expression of mouse Cebpe

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Cebp epsilon (Cebpe Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C3-PLK4-S305A-3xFLAG

   Plasmid

   #69839

   Purpose

   This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Use

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   S305A

   Promoter

   CMV

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mcherry Gamma 9 ---pcDNA3.1

   Plasmid

   #64203

   Purpose

   G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   G protein Gamma 9 (GNGT2 Human)
   

   Use

   Tags

   6xHis and mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pPN062

   Plasmid

   #91595

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPN063

   Plasmid

   #91596

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

   Plasmid

   #102986

   Purpose

   AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   membrane-targeted SNAP protein after FLP-mediated recombination
   Nuclear-targeted eGFP after FLP-mediated recombination
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2plus-mKate2

    Plasmid

    #162624

    Purpose

    Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23_Promoter_1-3

    Plasmid

    #210629

    Purpose

    Luciferase experiment for TAL1 promoter 1-3

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    promoter 1-3 (TAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-sgRELA

    Plasmid

    #83944

    Purpose

    Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgRELA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-pr-mKikGR1 (mKikGR1-V69T)

    Plasmid

    #99227

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mKikGR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T (Eos notatio…

    Promoter

    T7

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FH1-MGT#2-mCherry

    Plasmid

    #164097

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#2-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiex PKC - Peptide 8 mCit mCer

    Plasmid

    #83563

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER-hHSF2 shRNA

    Plasmid

    #118346

    Purpose

    This plasmid expresses shRNA against hHSF2.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF2 shRNA (HSF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130989

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 139H2_LC

    Plasmid

    #206202

    Purpose

    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. MDH1-shSHP2-3-H2Kb2

    Plasmid

    #17854

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shSHP2 (PTPN11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-hs-3xHA-delta6xNHL-LIN-41

    Plasmid

    #52722

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 593–868

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pCS3-MT-BX/p14ARF

    Plasmid

    #78761

    Purpose

    To overexpress p14ARF in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-mCherry-mCherry-FLARE-Cameleon

    Plasmid

    #123352

    Purpose

    Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid

    #79885

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL4.23_TSS1

    Plasmid

    #210633

    Purpose

    Luciferase experiment for TAL1 TSS1

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 TSS1 (TAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL4.23_TSS2

    Plasmid

    #210634

    Purpose

    Luciferase experiment for TAL1 TSS2

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 TSS2 (TAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL4.23_TSS4

    Plasmid

    #210635

    Purpose

    Luciferase experiment for TAL1 TSS4

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 TSS4 (TAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.23_TSS5

    Plasmid

    #210636

    Purpose

    Luciferase experiment for TAL1 TSS5

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 TSS5 (TAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80058

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Sumo3 PolQM1 deltains1

    Plasmid

    #78461

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2149-2170; Q2513R (please see…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only