We narrowed to 5,803 results for: SUP
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Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD250.2
Plasmid#125077PurposeExpression of torR under PLtetO-1, output promoter PtorCAD129DepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR300.3
Plasmid#125079PurposeExpression of uhpA under PLtetO-1, output Promoter PuhpT99DepositorAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0078
Plasmid#117645PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
KHBD00703
Plasmid#39739DepositorAvailable SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
MGAT-SEP-mRuby3
Plasmid#200937Purposecis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GALT-SEP-mRuby3
Plasmid#200938Purposetrans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyDock
Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,ā¦PromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
TagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasmā¦PromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-GFP
Plasmid#187641PurposeVHL Expression vector with GFPDepositorAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-Tdtomato
Plasmid#187643PurposeVHL Expression vector with TdtomatoDepositorAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-SEP-mRuby3
Plasmid#200939Purposeendolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_GFP 150TAG 6xHis
Plasmid#140015PurposePlasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
Tags6xHisExpressionMammalianMutation150TAGPromoterEF1Available SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stapā¦ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmTurqoise KIF1A shRNA
Plasmid#102849PurposeGenerates shRNA specific to Rat KIF1A along with cell fill pmTurqoiseDepositorAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SEP-Glur2 (MCA10)
Plasmid#64941Purposemammalian expression of rat GluR2DepositorAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS
Plasmid#140009PurposePlasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
TagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#69496PurposepJL1 expression vector with sfGFPDepositorInsertSuper folder GFP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS AF
Plasmid#140023PurposePlasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationY271A, Y349FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
VAMP4-SEP
Plasmid#174406PurposeReporter for VAMP4 exocytosis in mammalian cellsDepositorInsertvesicle associated membrane protein 4 (VAMP4 Human)
Tagssuperecliptic phulorin (mutated GFP)ExpressionMammalianPromoterCMVAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-Lats1
Plasmid#19053DepositorInsertLarge tumor suppressor kinase 1, N mutant (LATS1 Human)
TagsEGFPExpressionMammalianMutation**Available SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
hSOD1-FLAG
Plasmid#182922PurposeMammalian expression plasmid for human WT SOD1DepositorAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-GFP
Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
ExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Mb haloTyrRS C6
Plasmid#160378PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.DepositorInsertC6 HaloTyrosine tRNA synthatase
UseSynthetic BiologyExpressionBacterial and MammalianMutationL270S, Y271L, N311G, C313TAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(C162F)-3F-BirA
Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon optiā¦PromoterEF-1aAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mx1201 PylRS
Plasmid#154764Purposeplasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT sfGPF 150TAG
Plasmid#154766Purposeplasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, U25C in PylTPromoterEF1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only