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We narrowed to 1,273 results for: grna cloning vector

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Showing: 851 - 900 of 1273 results

  2. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  3. lifeact-mNeongreen-pA

    Plasmid
    #241202
    Purpose
    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen
    Depositor
    Junsu Kang
    Insert
    lifeact-mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241202-plasmid-map-sequence-id-482031

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  4. linker-P2A-mNeongreen-ORF1

    Plasmid
    #241176
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241176-plasmid-map-sequence-id-482017

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  5. linker-P2A-mNeongreen-ORF2

    Plasmid
    #241177
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241177-plasmid-map-sequence-id-482016

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  6. linker-P2A-mNeongreen-ORF3

    Plasmid
    #241178
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241178-plasmid-map-sequence-id-482014

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  7. FRT-acrymCherry-FRT

    Plasmid
    #241179
    Purpose
    Middle entry vector for mini-Golden subcloning platform; acry-mCherry
    Depositor
    Junsu Kang
    Insert
    acry-mCherry
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241179-plasmid-map-sequence-id-482012

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  10. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

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  11. SA-linker-P2A-mNeongreen-ORF1

    Plasmid
    #241208
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241208-plasmid-map-sequence-id-484613

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  12. SA-linker-P2A-mNeongreen-ORF2

    Plasmid
    #241209
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241209-plasmid-map-sequence-id-484611

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  13. SA-linker-P2A-mNeongreen-ORF3

    Plasmid
    #241210
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241210-plasmid-map-sequence-id-484600

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  14. nzCas9n-GSG-linker-P2A-mNeongreen

    Plasmid
    #241189
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241189-plasmid-map-sequence-id-482166

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  17. Banshee-ShCit-A

    Plasmid
    #85216
    Purpose
    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
    Depositor
    Alex Minella
    Article
    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002
    Insert
    shCit-A
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    H1
    Available Since
    Feb. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    85216-plasmid-map-sequence-id-171277

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  18. Banshee-ShCit-B

    Plasmid
    #85217
    Purpose
    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
    Depositor
    Alex Minella
    Article
    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002
    Insert
    shCit-B
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    H1
    Available Since
    Feb. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    85217-plasmid-map-sequence-id-171275

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  19. PB-2G-CNCB

    Plasmid
    #236764
    Purpose
    A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter, human U6 promoter, CAG promoter
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    236764-plasmid-map-sequence-id-473439

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  20. mCherry-P2A-NTRv2-pA

    Plasmid
    #241195
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2
    Depositor
    Junsu Kang
    Insert
    mCherry; NTR-v2
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241195-plasmid-map-sequence-id-482042

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  21. linker-P2A-mem-mScarlet-ORF-3

    Plasmid
    #241185
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mScarlet
    Depositor
    Junsu Kang
    Insert
    mScarlet
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    241185-plasmid-map-sequence-id-482164

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  22. linker-P2A-mem-mScarlet-ORF-2

    Plasmid
    #241184
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mScarlet
    Depositor
    Junsu Kang
    Insert
    mScarlet
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    241184-plasmid-map-sequence-id-482140

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  23. linker-P2A-mem-mScarlet-ORF-1

    Plasmid
    #241183
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mScarlet
    Depositor
    Junsu Kang
    Insert
    mScarlet
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    241183-plasmid-map-sequence-id-482141

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  24. linker-P2A-lifeact-mNeongreen-ORF-1

    Plasmid
    #241172
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241172-plasmid-map-sequence-id-482084

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  25. linker-P2A-lifeact-mNeongreen-ORF-2

    Plasmid
    #241173
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241173-plasmid-map-sequence-id-482034

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  26. linker-P2A-lifeact-mNeongreen-ORF-3

    Plasmid
    #241174
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241174-plasmid-map-sequence-id-482021

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  33. SA-linker-P2A-mem-mScarlet-ORF-2

    Plasmid
    #241187
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mScarlet
    Depositor
    Junsu Kang
    Insert
    mScarlet
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241187-plasmid-map-sequence-id-482179

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  34. SA-linker-P2A-mem-mScarlet-ORF-3

    Plasmid
    #241188
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mScarlet
    Depositor
    Junsu Kang
    Insert
    mScarlet
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241188-plasmid-map-sequence-id-482168

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  35. SA-linker-P2A-mem-mScarlet-ORF-1

    Plasmid
    #241186
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mScarlet
    Depositor
    Junsu Kang
    Insert
    mScarlet
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241186-plasmid-map-sequence-id-482157

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Showing: 851 - 900 of 1273 results