4,179 results

1. CMV-mito-LAR-GECO1.2

   Plasmid

   #61245

   Purpose

   Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1.2
   

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1.2: N45I/A47R/E1…

   Promoter

   CMV

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE YAP1

   Plasmid

   #15682

   Depositor

   Joan Brugge

   Article

   Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   YAP (YAP1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHYRS52

   Plasmid

   #31122

   Depositor

   Hideo Iwai

   Article

   Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

   Insert

   Ulp1 (ULP1 Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Available Since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. 413 pSG5L HA RB

   Plasmid

   #10720

   Depositor

   William Sellers

   Article

   Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

   Insert

   RB (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-myc-Rac1-Q61L

   Plasmid

   #12983

   Depositor

   Gary Bokoch

   Insert

   Rac1 constitutively active (RAC1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA2640

   Plasmid

   #25434

   Purpose

   Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   reverse tetracycline-regulated transactivator Advanced
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL-SIN-EOS-S(4+)-EiP

   Plasmid

   #21314

   Purpose

   Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EOS-S(4+) promoter region

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. E[beta]C

   Plasmid

   #24312

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Mutation

   Sites phosphorylated by GSK-3beta have been mutat…

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK7-FLAG-TSC1

   Plasmid

   #8995

   Depositor

   John Blenis

   Article

   Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

   Insert

   TSC1 (TSC1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonor_mU6

    Plasmid

    #69350

    Purpose

    sgRNA scaffold and mouse U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    mU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    mU6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lyn11-targeted FRB (LDR)

    Plasmid

    #20147

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    Lyn-linker-FRB (LDR) (MTOR Human)
    

    Expression

    Mammalian

    Available Since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-VHL wt-pBabe-puro

    Plasmid

    #19234

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR207 SARS-CoV-2 E

    Plasmid

    #141273

    Purpose

    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. 800 pSG5L HA PTEN wt

    Plasmid

    #10750

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. CKAR

    Plasmid

    #14860

    Depositor

    Alexandra Newton

    Article

    Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

    Insert

    C kinase activity reporter
    

    Tags

    CFP and YFP

    Expression

    Mammalian

    Available Since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC119-gRNA

    Plasmid

    #52255

    Purpose

    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    

    Use

    CRISPR; Plant expression

    Promoter

    Arabidopsis U6 polymerase III promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLOX-Ttag-iresTK

    Plasmid

    #12246

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    SV40 Large T, HSV1-TK (SV40gp6 Simian virus)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. Myc tag WT PTB

    Plasmid

    #23024

    Depositor

    Douglas Black

    Article

    Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

    Insert

    PTB1 (PTBP1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only