4,235 results

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pMedium-FT-N1

Plasmid

#31911

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-EGFR

Plasmid

#23935

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EGFR (EGFR Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-ACTR3

Plasmid

#8462

Depositor

Matthew Welch

Article

Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

Insert

ACTR3 (ACTR3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-mPIAS3

Plasmid

#15207

Depositor

Ke Shuai

Article

Chung et al Science. 1997 Dec 5. 278(5344):1803-5.

Insert

PIAS3 (Pias3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

V580I

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2Uw-2

Plasmid

#26227

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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APEX2 - Tubulin in pEGFP

Plasmid

#66171

Purpose

APEX2 fused to the N-terminus of alpha tubulin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - alpha tubulin

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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C5V

Plasmid

#26394

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-5-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pSECC

Plasmid

#60820

Purpose

3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

Depositor

Tyler Jacks

Article

Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

Inserts

Cas9
Cre

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Mammalian

Mutation

BsmBI site eliminated by C->A; E308G

Promoter

EFS and EFS (after Cas9-2A)

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

Plasmid

#20299

Purpose

Cre-activated AAV expression of mCherry

Depositor

Karl Deisseroth

Has Service

AAV9

Insert

mCherry

Use

AAV

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-mApg16L

Plasmid

#24302

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

Insert

autophagy related genes 16L (Atg16l1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V583M

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-wGBD

Plasmid

#26734

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

GFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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Myc-PSF-WT

Plasmid

#35183

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pEB-Tg

Plasmid

#28220

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Insert

SV40 large T

Expression

Mammalian

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL5-FLAG

Plasmid

#15823

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin5 (CUL5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N441

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4B-FLAG

Plasmid

#15822

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4B (CUL4B Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N594

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pDONR223-JAK3

Plasmid

#23944

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

JAK3 (JAK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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VP16-ER alpha

Plasmid

#11351

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER alpha (ESR1 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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