4,143 results

1. p2xFLAGhYAP1

   Plasmid

   #17791

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-Puro

   Plasmid

   #141388

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf7a (ORF7a SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCMVTAG-NEMO

   Plasmid

   #11970

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pG14-MS2-GFP

   Plasmid

   #27117

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2-binding protein
   

   Use

   Tags

   GFP, HA, and NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-DRD2

   Plasmid

   #24099

   Depositor

   Jean-Michel Arrang

   Article

   Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

   Insert

   DRD2 (DRD2 Human)
   

   Use

   Tags

   alpha7 nicotinic signal peptide and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-link-yomRuby2-SpHis5

   Plasmid

   #44858

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-EGFP-AID-BubR1

   Plasmid

   #47330

   Purpose

   for plasmid integration using the Flp-In system. AID at N-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
   

   Use

   Tags

   AID and GFP

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Gateway

   Plasmid

   #32671

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Type

   Empty backbone

   Use

   AAV; Aav

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. human oct4-GFP

    Plasmid

    #21153

    Depositor

    Rudolf Jaenisch

    Article

    Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

    Insert

    hOCT4-GFP (POU5F1 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Duet011

    Plasmid

    #17627

    Depositor

    Linzhao Cheng

    Article

    Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

    Insert

    UBC promoter - GFP - PGK promoter - Hygro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3 NSE promoter

    Plasmid

    #11606

    Depositor

    Rosalyn Adam

    Article

    Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

    Insert

    NSE promoter (ENO2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBiFC-bJunVN155(I152L)

    Plasmid

    #27098

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    bJunVN155(I152L) (Jun Rat, and Aequorea Victoria)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L in Venus(1-154)

    Promoter

    Available since

    Jan. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mTOR_2 shRNA

    Plasmid

    #1856

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    mTOR (MTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51085

    Purpose

    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO/cmycDrosha

    Plasmid

    #10828

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

    Insert

    Drosha (DROSHA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. V405 4HA-CKIe

    Plasmid

    #13724

    Depositor

    David Virshup

    Article

    Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

    Insert

    casein kinase 1 epsilon (CSNK1E Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223-ALK

    Plasmid

    #23917

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ALK (ALK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3xIRS luciferase

    Plasmid

    #13511

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    3xIRS (IGFBP1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge