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Plasmid#144810PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSP100 (SP100 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG
Plasmid#199328Purposemutated RHIM motive (interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged V458, Q459,V460 and G461 to alaninePromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPANI2 synthetic promoter carrying binding sites f…Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NECTIN3-COMP5AP-AviTag-9xHis
Plasmid#157355PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertNECTIN3 (NECTIN3 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PDCD1LG2-Fc(DAPA)-AviTag-6xHis
Plasmid#156612PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPDCD1LG2 (PDCD1LG2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD-S32
Plasmid#140303PurposeExpresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationlacks the KD regionPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-STK19
Plasmid#116795PurposeLentiviral expression of STK19DepositorInsertSTK19 (STK19 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.4-AP-His
Plasmid#72044PurposeExpresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6d.4 (Sema6d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.Luc.IRES.EGFP.SV40
Plasmid#105533PurposeAAV expression of Luciferase and EGFP from CMV promoterDepositorInsertLuciferase
UseAAV and LuciferaseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MmXYRS-4xM15
Plasmid#155343PurposePlasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.DepositorInsertHumanized MmXYRS/tRNAM15
UseTagsExpressionMammalianMutationPromoterCMV/U6Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst44-GFP
Plasmid#172310PurposeSST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneuronsDepositorInsertSst44
UseAAVTagsExpressionMutationPromoterpB-GlobinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Frb-EGFP-HOTag6
Plasmid#106919PurposeExpress Frb-EGFP-HOTag6 fusion in mammalian cells.DepositorInsertFrb-EGFP-HOTag6
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLI50
Plasmid#13573DepositorTypeEmpty backboneUseS. aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Cre
Plasmid#123128PurposeBacterial expression plasmid for wild-type Cre with an N-terminal His-tag.DepositorInsertCre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)
Plasmid#217306PurposesgRNA mcherry + PuromycinDepositorInsertpU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
UseCRISPRTagsExpressionMutationPromoterU6, Ef1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab11a-7
Plasmid#55124PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab11a (RAB11A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C1)-PP1alpha
Plasmid#44224DepositorInsertPP1A (PPP1CA Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXPR_071
Plasmid#164558PurposeHigher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro SDF-1 alpha
Plasmid#12270DepositorInsertSDF-1 alpha (CXCL12 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEEV2/11
Plasmid#166888PurposeHelper/packaging plasmid for production of AAV2/11 pseudotype vectorDepositorInsertAAV2 rep/AAV11 cap
UseAAVTagsExpressionMutationPromoterAAV2 p5Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::lacZ-H11
Plasmid#139099PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHsp68Available sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP670
Plasmid#136571PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertH2B-emiRFP670
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-E1b:eGFP-Ds
Plasmid#102417PurposeFluorescent vector for screening enhancer elements in zebrafishDepositorInsertE1b:eGFP
UseTagsExpressionBacterialMutationPromoterZebrafish E1b minimal promoterAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only