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We narrowed to 9,971 results for: Gnas

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Showing: 9001 - 9050 of 9971 results

  2. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

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  3. RAMA-bio

    Plasmid
    #47786
    Purpose
    Expresses enzymatically monobiotinylated full-length RAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RAMA
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47786-plasmid-map-sequence-id-68822

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  4. 3xFN-Tel-TAL/pMXs-puro

    Plasmid
    #63590
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Promoter
    LTR
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63590-plasmid-map-sequence-id-108379

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  8. MSP9-bio

    Plasmid
    #47740
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP9 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP9
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47740-plasmid-map-sequence-id-68875

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  12. P12-bio

    Plasmid
    #47725
    Purpose
    Expresses enzymatically monobiotinylated full-length P12 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised P12
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47725-plasmid-map-sequence-id-68459

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  17. CLAG3.2-bio

    Plasmid
    #47797
    Purpose
    Expresses enzymatically monobiotinylated full-length CLAG3.2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised CLAG3.2
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47797-plasmid-map-sequence-id-68803

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  19. ASP-bio

    Plasmid
    #47745
    Purpose
    Expresses enzymatically monobiotinylated full-length ASP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised ASP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47745-plasmid-map-sequence-id-68757

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  20. RAP1-bio

    Plasmid
    #47794
    Purpose
    Expresses enzymatically monobiotinylated full-length RAP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RAP1
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47794-plasmid-map-sequence-id-68800

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  21. RhopH2-bio

    Plasmid
    #47798
    Purpose
    Expresses enzymatically monobiotinylated full-length RhopH2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RhopH2
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47798-plasmid-map-sequence-id-68809

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  22. pNUT N6His K206E hTFNG

    Plasmid
    #70130
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    He et al Biochemistry. 1999 Jul 27;38(30):9704-11
    Insert
    mutated human serum transferrin (TF Human)
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid, Asn413Asp, Asn…
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70130-plasmid-map-sequence-id-125031

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  25. Myc-delta/gamma2SIL

    Plasmid
    #119731
    Purpose
    GABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRD/GABRG2 (Gabrg2, Gabrd Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    119731-plasmid-map-sequence-id-229623

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  28. pBT3047

    Plasmid
    #122547
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)
    Tags
    MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122547-plasmid-map-sequence-id-238211

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  29. H101-bio

    Plasmid
    #47733
    Purpose
    Expresses enzymatically monobiotinylated full-length H101 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised H101
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47733-plasmid-map-sequence-id-68566

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  30. PTRAMP-bio

    Plasmid
    #47793
    Purpose
    Expresses enzymatically monobiotinylated full-length PTRAMP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PTRAMP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47793-plasmid-map-sequence-id-68770

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  32. pAIDA_TEV_fap2EC

    Plasmid
    #246866
    Purpose
    Expression of Fusobacterium nucleatum ATCC23726 Fap2 extracellular domain (42-3271) with histag and TEV site in fusion with E. coli autotransporter AIDA
    Depositor
    Daniel Roderer
    Article
    Schopf et al Nat Commun. 2025 Aug 29;16(1):8104. doi:
    Insert
    F. nucleatum ATCC23726 Fap2 (extracellular domain, res. 42–3271)
    Tags
    8x histidine, AIDA autotransporter, AIDA signal s…
    Expression
    Bacterial
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    246866-plasmid-map-sequence-id-493107

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  35. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-moxBFP

    Plasmid
    #242410
    Purpose
    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (vaccinia virus E3, Toscana virus NSs, Theiler’s virus L*) and moxBFP (in place of inflammatory signaling inhibitors).
    Depositor
    Ewan St. John Smith
    Article
    Lim et al bioRxiv 2024.09.24.614636
    Inserts
    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-moxBFP
    Use
    Mouse Targeting; Self-amplifying rna
    Expression
    Mammalian
    Available Since
    Sept. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242410-plasmid-map-sequence-id-487193

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  36. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα

    Plasmid
    #242411
    Purpose
    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).
    Depositor
    Ewan St. John Smith
    Article
    Lim et al bioRxiv 2024.09.24.614636
    Inserts
    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-srIκBα
    Use
    Mouse Targeting; Self-amplifying rna
    Expression
    Mammalian
    Mutation
    serines 32 and 36 to alanines
    Available Since
    Sept. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242411-plasmid-map-sequence-id-487192

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  39. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202042
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-1xNLS-[Activ]
    Expression
    Mammalian
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202042-plasmid-map-sequence-id-401647

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  40. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202043
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-[Activ]
    Expression
    Mammalian
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202043-plasmid-map-sequence-id-401604

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  41. pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA

    Plasmid
    #202044
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-VP64
    Expression
    Mammalian
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202044-plasmid-map-sequence-id-401598

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  45. pYI2294

    Plasmid
    #228352
    Purpose
    Methanol-inducible ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    KpAOX1 promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228352-plasmid-map-sequence-id-457091

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  46. pYI2292

    Plasmid
    #228350
    Purpose
    Methanol-inducible ALX-0171 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    KpAOX1 promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228350-plasmid-map-sequence-id-457095

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  47. pYI2293

    Plasmid
    #228351
    Purpose
    Constitutive ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228351-plasmid-map-sequence-id-457078

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  48. pMT1461

    Plasmid
    #228319
    Purpose
    Doxycycline-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    Doxycycline-regulatable synthetic promoter (KpTc7…
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228319-plasmid-map-sequence-id-457683

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Showing: 9001 - 9050 of 9971 results