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We narrowed to 7,778 results for: CCH

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Showing: 7321 - 7360 of 7778 results

  7. kanMX6-ins2

    Plasmid
    #195039
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195039-plasmid-map-sequence-id-384242

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  9. pC-SUR-YR

    Plasmid
    #61768
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Sur gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Promoter
    Magnaporthe oryzae ILV promoter
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61768-plasmid-map-sequence-id-442790

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  10. natMX6-ins5

    Plasmid
    #195042
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195042-plasmid-map-sequence-id-384245

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  11. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105983
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    SYFP2-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Promoter
    human Synapsin 1
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105983-plasmid-map-sequence-id-235283

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  12. TRP1MX6-ins4

    Plasmid
    #195041
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    TRP1
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195041-plasmid-map-sequence-id-384244

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  16. Lyn-FLINC-EKAR1

    Plasmid
    #87707
    Purpose
    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87707-plasmid-map-sequence-id-171091

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  17. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid
    #127483
    Purpose
    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Joel Malek
    Article
    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1
    Insert
    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    Expression
    Bacterial
    Mutation
    One nucleotide inserted between lambda CI and LGF…
    Available Since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127483-plasmid-map-sequence-id-262924

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  18. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105982
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    FAPdL5-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Promoter
    human synapsin 1
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105982-plasmid-map-sequence-id-235274

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  19. pZE21/UBP1/ClpS

    Plasmid
    #98566
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    In a synthetic operon downstream of UBP1 and Remo…
    Promoter
    Same as UBP1 (operon) and Tet promoter (aTc induc…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98566-plasmid-map-sequence-id-203067

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  20. Coexpressed Free GFP-NES

    Plasmid
    #182491
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    GFP-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182491-plasmid-map-sequence-id-360449

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  21. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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  27. pJTB115

    Plasmid
    #248909
    Purpose
    Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Article
    Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-
    Inserts
    SMK1 Mitogen-activated Protein Kinase (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes, Budding Yeast)
    Ssp2 (SSP2 Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form, Budding Yeast)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    NdeI and NcoI sites were removed for cloning purp…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248909-plasmid-map-sequence-id-497710

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  28. pAvA139

    Plasmid
    #248145
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen1' mutations
    Depositor
    Emil Jensen
    Article
    van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s
    Insert
    luxR (gen1 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: N24K, P41 (CCA→CCG), N46D, T92S, V98 (GT…
    Promoter
    pPGK1
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248145-plasmid-map-sequence-id-497174

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  33. pYO391

    Plasmid
    #235751
    Purpose
    Protein expression of ScVPS38, untagged + ScVPS34, untagged + FL ScVPS15-ZZ + ZZ-FL ScVPS30
    Depositor
    Roger Williams
    Article
    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d
    Inserts
    VPS34 (VPS34 Budding Yeast)
    VPS15 (VPS15 Budding Yeast)
    VPS38 (VPS38 Budding Yeast)
    VPS30 (VPS30 Budding Yeast)
    Tags
    3xTEV-ZZ and ZZ-3xTEV
    Expression
    Yeast
    Mutation
    S2A and T134A, I851R
    Promoter
    GALGAPDH
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    235751-plasmid-map-sequence-id-490579

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  36. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS

    Plasmid
    #221117
    Purpose
    FAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-Tailless
    Expression
    Yeast
    Mutation
    STE3 mutant that lacks the entire C-terminal tail…
    Promoter
    STE3
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    221117-plasmid-map-sequence-id-466649

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  38. pAM88

    Plasmid
    #226680
    Purpose
    Rpt1I191TAG base (3xFLAG-Rpt1I191TAG, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5, Rpt6)
    Depositor
    Andreas Martin
    Article
    Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi
    Inserts
    rpt1 (RPT1 Budding Yeast)
    Rpt2 (RPT2 Budding Yeast)
    Rpt3 (RPT3 Budding Yeast)
    Rpt4 (RPT4 Budding Yeast)
    Rpt5 (RPT5 Budding Yeast)
    Rpt6 (RPT6 Budding Yeast)
    Tags
    FLAG and His
    Expression
    Bacterial
    Mutation
    I191TAG
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    226680-plasmid-map-sequence-id-450922

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Showing: 7321 - 7360 of 7778 results