We narrowed to 159,258 results for: Gene
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Plasmid#220903PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8923
Plasmid#220904PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8932
Plasmid#220906PurposeMobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)DepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 DdCBE A1 (DddA6-C)
Plasmid#221992PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: T1413IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 DdCBE T2 (DddA6-N)
Plasmid#221993PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: Q1310R, S1330I, T1380IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE T2 (DddA6-N)
Plasmid#221995PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…PromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE G2
Plasmid#221959PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A, See depositor commentsPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-eC3GFP
Plasmid#225485PurposeTet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFPDepositorInsertExoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251, See Depositor CommentsPromoterHuman ubiquitin CAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB
Plasmid#225488PurposeTet-on lentiviral vector for controlled expression of GDNFDepositorInsertTetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
UseLentiviralTagsExpressionMammalianMutationPromoterMouse phosphoglycerate kinase 1Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM028
Plasmid#216809PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM030
Plasmid#216810PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM068
Plasmid#216811PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
UseTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSX2739
Plasmid#200765PurposeExpresses mKate2-tag fused CDC-42 with 8xMS2(V1) stem loops in C. elegans, and the tissue-specific promoter col-19 is used.DepositorInsert8xMS2
UseTagsmKate2ExpressionWormMutationPromotercol-19Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP ∆101-140
Plasmid#221212PurposeMammalian expression vector of triple Flag tagged NFATC2IP with amino acids 101-140 deletedDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutation1st Methionine and amino acids 101-140 deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3XFLAG-NFATC2IP SLD2
Plasmid#221388PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 2 (SLD2, aa 345-418)DepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutationAmino acids 1-344 of NFATC2IP deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-eGFP-NFAT2CIP ∆101-140
Plasmid#221437PurposeMammalian expression vector of eGFP tagged NFATC2IP with amino acids 101-140 deletedDepositorInsertNFATC2IP (NFATC2IP Human)
UseTagseGFPExpressionMammalianMutation1st Methionine and amino acids 101-140 deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-eGFP-NFATC2IP SLD2
Plasmid#221438PurposeMammalian expression vector of eGFP tagged NFATC2IP SUMO-like domain 2 (SLD2, aa 345-418)DepositorInsertNFATC2IP (NFATC2IP Human)
UseTagseGFPExpressionMammalianMutationAmino acids 1-344 of NFATC2IP deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
GB3473
Plasmid#215246PurposeCDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathwayDepositorInsertLuz
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMVd1-NLS-GAL4-iLight-VP16
Plasmid#219549PurposeNLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight-VP16
UseAAVTagsExpressionMammalianMutationPromoterCMVd1Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MGS0156_pET29b
Plasmid#215410PurposeExpression construct for MGS0156 gene, codon optimized for expression in E. coliDepositorInsertMGS0156
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIDAF
Plasmid#213708PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIBY55
Plasmid#213707PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC Destination
Plasmid#207675PurposeTrio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterCymRC, PhlF, VanCCAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk18
Plasmid#209000PurposeRiboprobe generationDepositorInsertkcnk18 (kcnk18 Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk2a
Plasmid#208983PurposeRiboprobe generationDepositorInsertkcnk2a (kcnk2a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk6
Plasmid#208990PurposeRiboprobe generationDepositorInsertkcnk6 (kcnk6 Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk13a
Plasmid#208996PurposeRiboprobe generationDepositorInsertkcnk13a (kcnk13a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cc81-mut
Plasmid#199745PurposeExpresses AAV2 Rep and AAV.cc81 CapDepositorInsertAAV.cc81 Cap
UseAAVTagsExpressionMammalianMutationmutated amino acids 586-592 in CapPromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cc84-mut
Plasmid#199746PurposeExpresses AAV2 Rep and AAV.cc84 CapDepositorInsertAAV.cc84 Cap
UseAAVTagsExpressionMammalianMutationmutated amino acids 586-592 in CapPromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk3
Plasmid#207614PurposeZebrafish Alk3 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)
UseTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKP2del4
Plasmid#130837PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of terminal four amino acid residuesPromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP2delKASH
Plasmid#130838PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domain, addition of three alani…PromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP1del4ec
Plasmid#130844PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of terminal four amino acid residuesPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP1delKASHec
Plasmid#130845PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of KASH domain, addition of three alanin…PromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMB1-C-TelR
Plasmid#204981PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1-D-TelR
Plasmid#204982PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1-E-TelR
Plasmid#204984PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB2-B-TelR
Plasmid#204988PurposeLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB2-C-TelR
Plasmid#204989PurposeLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB2-D-TelR
Plasmid#204990PurposeLevel 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0018
Plasmid#205587Purposep3flag_HygR(1-52)-NpuDnaE(N)DepositorInsertHygR-NpuDnaE(N)_homo-codon-OP
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015
Plasmid#202024PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
UseTagsExpressionBacterialMutationGvpA T6A-L40APromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Silene_conica _GlnRS_MitoTP_pK7FWG2
Plasmid#202654PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _LysRS_MitoTP_pK7FWG2
Plasmid#202655PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Agrostemma_githago _LysRS_MitoTP_pK7FWG2
Plasmid#202656PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _TyrRS_MitoTP_pK7FWG2
Plasmid#202657PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_vulgaris _TyrRS_MitoTP_pK7FWG2
Plasmid#202658PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _PheRS_MitoTP_pK7FWG2
Plasmid#202659PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Silene_conica _PheRS_PlastidTP_pK7FWG2
Plasmid#202660PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits