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177,170 results

Showing: 9301 - 9350 of 177170 results

  11. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid
    #117006
    Purpose
    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Publication
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    117006-plasmid-map-sequence-id-227036

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  13. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    113193-plasmid-map-sequence-id-216317

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  15. pAAV-Ef1a-fDIO mCherry

    Plasmid
    #114471
    Purpose
    Flp-dependent mCherry Control
    Depositor
    Karl Deisseroth
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde
    Insert
    mCherry
    Use
    AAV
    Promoter
    Ef1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    36 Articles
    114471-plasmid-map-sequence-id-220928

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  17. pALOD4

    Plasmid
    #111026
    Purpose
    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
    Depositor
    Arun Radhakrishnan
    Publication
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A
    Promoter
    T7
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    111026-plasmid-map-sequence-id-214044

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  18. pSEMS-Tom20-Halo7Tag

    Plasmid
    #111135
    Purpose
    Tom20 with selflabeling tag, e.g. for fluorescence microscopy
    Depositor
    Karin Busch
    Publication
    Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
    Tags
    Halo7 Tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    111135-plasmid-map-sequence-id-213894

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  26. FRB-mCherry-FTH1

    Plasmid
    #100749
    Purpose
    Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag
    Depositor
    Stephen Royle
    Publication
    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ferritin heavy chain 1 (FTH1 Human)
    Tags
    FRB and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    100749-plasmid-map-sequence-id-194021

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  27. pX330a dCas9-KRAB

    Plasmid
    #92361
    Purpose
    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    92361-plasmid-map-sequence-id-178231

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  28. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid
    #99280
    Purpose
    Can be used to generate AAV virus that will express mScarlet in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mScarlet
    Use
    AAV and Cre/Lox
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    99280-plasmid-map-sequence-id-487711

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  29. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
    91565-plasmid-map-sequence-id-456463

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  34. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86551-plasmid-map-sequence-id-171421

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  35. pCMV-SPORT6-hHMGCR1

    Plasmid
    #86085
    Purpose
    expresses the human HMG-CoA reductase isoform 1
    Depositor
    Anne Galy
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human HMG-CoA Reductase isoform 1 (HMGCR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles

  40. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84240-plasmid-map-sequence-id-160812

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  42. Grp1-PH pEGFP-C1

    Plasmid
    #71378
    Purpose
    For analysis of PH domain localization in mammalian cells
    Depositor
    Mark Lemmon
    Publication
    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Grp1-PH (Cyth3 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin homology (PH) domain; aa 247-399
    Promoter
    CMV
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    71378-plasmid-map-sequence-id-422685

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  43. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    gfap
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    75024-plasmid-map-sequence-id-450679

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Showing: 9301 - 9350 of 177170 results