164,425 results

1. pCS2-nCas9n

   Plasmid

   #47929

   Purpose

   expression of an optimized Cas9 for genome-editing in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   nls-zcas9-nls
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Use

   Tags

   GFP11x7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDH-3xFLAG-hTCAB1-puroR

   Plasmid

   #167459

   Purpose

   3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells

   Depositor

   Steven Artandi

   Article

   Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

   Insert

   TCAB1 (WRAP53 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

   Plasmid

   #112022

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha/beta into TRBC1 HDRDT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDUP 4-1

   Plasmid

   #23022

   Depositor

   Douglas Black

   Article

   Modafferi et al Mol Cell Biol. 1997 Nov . 17(11):6537-45

   Insert

   c-src (Src Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-ABCB10-ChR2-eYFP

   Plasmid

   #206037

   Purpose

   Express ChR2 in mitochondria

   Depositor

   Lufang Zhou

   Article

   Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

   Insert

   ChR2
   

   Use

   Tags

   ABCB10 mitochondrial leading sequence and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tdTomato-Zyxin-6

   Plasmid

   #58148

   Purpose

   Localization: Focal Adhesions, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLVX-NPM1c-IRES-mCherry

   Plasmid

   #131825

   Purpose

   Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETDuet1_His-TEV-EGFP

   Plasmid

   #223723

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   EGFP, not monomeric (has A207)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Mustard Weed, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mito-mScarlet

    Plasmid

    #141153

    Purpose

    Fluorescent labeling of the mitochondrial matrix in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    COX4 (COX4 Budding Yeast)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-FLAG-S6K1

    Plasmid

    #100509

    Purpose

    Expresses FLAG tagged S6K1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

    Plasmid

    #187454

    Purpose

    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDonor-GDH

    Plasmid

    #140629

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    Mini-Tn6677, T7lac-GDH gene cassette
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RSV-Flag-Brd2

    Plasmid

    #86614

    Purpose

    Flag tagged mouse Brd2 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd2 (Brd2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3/Flag-METTL14

    Plasmid

    #53740

    Purpose

    For expression of METTL14 (methyltransferase-like 14) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 14 (METTL14 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSUMO3

    Plasmid

    #52260

    Purpose

    Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-3 (SUMO3 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLHCX-FLAG-mPKM2

    Plasmid

    #44239

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

    Insert

    Pyruvate Kinase M2 (Pkm Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCS2-SauCas9

    Plasmid

    #174040

    Purpose

    In vitro transcription of SauCas9 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    SauCas9
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiV_Neo_SIK3

    Plasmid

    #108103

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSUMO2

    Plasmid

    #52259

    Purpose

    Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pClneoMyc-LATS1

    Plasmid

    #66851

    Purpose

    Express Myc-tagged LATS1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS1 (LATS1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P44L mutation in LATS1 compared to GenBank refere…

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-6xGLY-3xFLAG-HIS3MX6

    Plasmid

    #20753

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AICSDP-47: CTNNB1-mEGFP

    Plasmid

    #109119

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pAKgfp1

    Plasmid

    #14076

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

26. FpG5

    Plasmid

    #69443

    Purpose

    lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mVenus C1

    Plasmid

    #27794

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus C1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6/V5-p53_R280K

    Plasmid

    #22933

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    R280K mutant

    Promoter

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pLKO_FOXA1_#2

    Plasmid

    #70096

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNF02-mJuniper

    Plasmid

    #219398

    Purpose

    Constitutive expression of mJuniper in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mJuniper
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMDlg/pRRE-int-MS2M

    Plasmid

    #166032

    Purpose

    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Insert

    Gag-Pol-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D64V mutation within the integrase within Pol

    Promoter

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. APEX2-V5-G3BP1_pTRE-G418

    Plasmid

    #202013

    Purpose

    expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    APEX2-V5-G3BP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-Tight

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTnsABC

    Plasmid

    #160031

    Purpose

    Expresses V. cholerae TnsABC

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    no

    Promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1A eIF4A3

    Plasmid

    #83035

    Purpose

    mammalian expression of eIF4A3

    Depositor

    Gideon Dreyfuss

    Article

    Chan et al RNA. 2004 Feb;10(2):200-9.

    Insert

    eIF4A3 (EIF4A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

35. esmGFP-C10-pVTCB052

    Plasmid

    #231303

    Purpose

    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

    Depositor

    Alexander Rives

    Insert

    esmGFP-C10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mito-meGFP

    Plasmid

    #172481

    Purpose

    meGFP targeted to the mitochondrial matrix with a human COX8 presequence

    Depositor

    Thomas Schwarz

    Insert

    Mito
    

    Use

    Tags

    meGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FLAG-Skp2

    Plasmid

    #89142

    Purpose

    mammalian expression of human Skp2 with N-terminal FLAG tag

    Depositor

    Elizabeth Wilson

    Article

    Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

    Insert

    Skp2 (SKP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pRSF-KpAGO207-1251

    Plasmid

    #117854

    Purpose

    Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

    Depositor

    Kotaro Nakanishi, Dinshaw Patel

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Argonaute
    

    Use

    Tags

    6xHis-SUMO encoded in vector

    Expression

    Bacterial

    Mutation

    missing first 206 amino acid residues

    Promoter

    T7

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHT-ads

    Plasmid

    #47382

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Insert

    ads
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac

    Available Since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSEVA23-GG

    Plasmid

    #208013

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    14G

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. human pirh2-(1-261)

    Plasmid

    #24878

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

45. pBT372

    Plasmid

    #125613

    Purpose

    Expresses evoCDA1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoCDA1-BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F23S A123V I195F

    Promoter

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCherryGFP-CoV2-Inframe-stop

    Plasmid

    #177620

    Purpose

    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCherryGFP-Inframe-noFSE

    Plasmid

    #177619

    Purpose

    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pC1-SypHer3s-dmito

    Plasmid

    #108119

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-dmito
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. phU1-CasRx-non-targeting (pKW82)

    Plasmid

    #229050

    Purpose

    Cloning backbone for CasRx crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. PcDNA3.1-2Strep

    Plasmid

    #210343

    Purpose

    Empty Vector for molecular cloning

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    2Strep tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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