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178,151 results

Showing: 3741 - 3760 of 178151 results

  1. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Publication
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105829-plasmid-map-sequence-id-203267

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  3. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Publication
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mClY-N1
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90457-plasmid-map-sequence-id-178580

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  4. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Publication
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83890-plasmid-map-sequence-id-240902

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  12. pAS1NB m Rosella I

    Plasmid
    #71247
    Purpose
    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.
    Depositor
    Mark Prescott
    Publication
    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20
    Insert
    Rosella
    Tags
    yeast mitochondrial targeting sequence of citrate…
    Expression
    Yeast
    Promoter
    PGK1
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    71247-plasmid-map-sequence-id-363461

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  16. pcDNA3-TORCAR(T/A)

    Plasmid
    #64928
    Purpose
    Negative-control mutant for TORCAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR(T/A) (EIF4EBP1 Human, Synthetic)
    Tags
    Cerulean and YPet
    Expression
    Mammalian
    Mutation
    4EBP1 domain contains T37A and T46A mutations.
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64928-plasmid-map-sequence-id-109685

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Showing: 3741 - 3760 of 178151 results