We narrowed to 6,963 results for: RAP

1. pCMS-eGFP-RTP801

   Plasmid

   #65057

   Purpose

   Expression of RTP801 in mammalian cells

   Depositor

   Cristina Malagelada, Lloyd Greene

   Article

   Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

   Insert

   DDIT4 (Ddit4 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLentiDP1.3

   Plasmid

   #171096

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Emerald, cdt1, miniAID, mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

   Plasmid

   #176027

   Purpose

   construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   rtTA3
   Luc2
   mtagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-MED4

   Plasmid

   #15425

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED4 (MED4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pLX304-MARK3_T211A

   Plasmid

   #107236

   Purpose

   Lentiviral gene expression vector for phospho-MARK3_T221A expression

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiDP1.2

   Plasmid

   #171095

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Emerald, Geminin, miniAID, mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hRedOpsin-fCD200

   Plasmid

   #164425

   Purpose

   AAV plasmid expressing full-length CD200 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

   Insert

   CD200 (Cd200 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human red opsin

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPC-FLAG.Mm.DMAP1

   Plasmid

   #184650

   Purpose

   Retroviral expression of mouse DMAP1

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   Dmap1 (Dmap1 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST27-GST-hMSN

   Plasmid

   #211823

   Purpose

   Express human MSN in mammalian cells

   Depositor

   Haian Fu

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Use

   Tags

   GST tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLC-RFP657-CASP8

    Plasmid

    #75164

    Purpose

    LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    CASP8 sgRNA (CASP8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCytERM-mGreenLantern

    Plasmid

    #164467

    Purpose

    Visualization of the ER in cells (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

    Plasmid

    #33255

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and phenylalan…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-MED27

    Plasmid

    #15424

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED27 (MED27 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pBMN mCherry-p62(W340A/ΔUBD)

    Plasmid

    #119688

    Purpose

    For mammalian expression of LIR mutant p62 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    W340A & UBD deletion (1-385)

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-shGBP1.1.mKO2

    Plasmid

    #85209

    Purpose

    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

    Plasmid

    #120296

    Purpose

    AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    Rosa26 gRNA (SpCas9 scaffold)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEGFP-N1-NF2

    Plasmid

    #166473

    Purpose

    Expresses fusion of mEGFP and NF2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, NF2 (NF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVAX1-BMP2/7 -

    Plasmid

    #137912

    Purpose

    Constitutive mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)

    Depositor

    Georg Feichtinger

    Article

    Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

    Inserts

    BMP2 (BMP2 Human)
    BMP7 (BMP7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. H517 SONIC HRASV12 donor

    Plasmid

    #138177

    Purpose

    CRISPR SONIC: HRAS G12V donor plasmid

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    HRASV12 (HRAS Human)
    

    Use

    Nhej donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pMX-IY LC3A

    Plasmid

    #89290

    Purpose

    For expression of the mammalian Atg8 protein LC3A without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3A (MAP1LC3A Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL-p21UTRm1&2

    Plasmid

    #20880

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pDEST-CMV GFP-LC3B G120

    Plasmid

    #123116

    Purpose

    Expresses EGFP-LC3B G120 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G

    Plasmid

    #194459

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ND4-FusXTBE-Left TALE

    Plasmid

    #196496

    Purpose

    Expresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ND4-FusXTBE-Right TALE

    Plasmid

    #196497

    Purpose

    Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-RNF8-Middle-part

    Plasmid

    #64672

    Purpose

    Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    ring finger protein 8 (RNF8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Containing only middle part of RNF8 amino acids 1…

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge