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Showing: 901 - 950 of 2919 results

  2. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

    Plasmid
    #196885
    Purpose
    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    196885-plasmid-map-sequence-id-391918

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  3. pCAG-tdTHC

    Plasmid
    #112621
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112621-plasmid-map-sequence-id-222036

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  4. Lenti-SAM-Cre

    Plasmid
    #198714
    Purpose
    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pgk and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198714-plasmid-map-sequence-id-402628

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  6. LeGO-iBSD-5LO-d4

    Plasmid
    #182743
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 4 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182743-plasmid-map-sequence-id-367810

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  7. LeGO-iBSD-5LO-p12

    Plasmid
    #182744
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform p12 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182744-plasmid-map-sequence-id-367805

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  8. LeGO-iBSD-5LO-E376Q

    Plasmid
    #182742
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase E376Q (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    enzymatically inactive 5-LO E376Q mutant, codon o…
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182742-plasmid-map-sequence-id-367803

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  9. LeGO-iBSD-5LO-d13

    Plasmid
    #182745
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 13 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182745-plasmid-map-sequence-id-367797

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  10. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    28306-plasmid-map-sequence-id-162239

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  11. miR-124

    Plasmid
    #182363
    Purpose
    Plasmid with 4x miR-124 target sites
    Depositor
    Mario Capecchi
    Insert
    4x miR-124 target sites
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182363-plasmid-map-sequence-id-358192

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  12. pAAV-synP-FLEX-TVA66T-EGFP-B19G

    Plasmid
    #64097
    Purpose
    Expresses TVA66T-EGFP-B19G under the synapsin promotor
    Depositor
    Ian Wickersham
    Insert
    TVA66T-EGFP-B19G
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    synapsin
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    64097-plasmid-map-sequence-id-107238

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  13. pBCSK_LEU2_I

    Plasmid
    #109060
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes
    Depositor
    Colin Logie
    Insert
    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109060-plasmid-map-sequence-id-209546

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  14. AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA

    Plasmid
    #223843
    Purpose
    Enhancer-driven expression of the iCre(R297T) recombinase in thalamic PVT neurons
    Depositor
    Bosiljka Tasic
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    Argenine in aa297 was replaced with Threonine
    Promoter
    minBG
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    223843-plasmid-map-sequence-id-443652

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  15. pAAV hSyn GFP-Cre

    Plasmid
    #175381
    Purpose
    expresses GFP-Cre under the control of a human synapsin promoter
    Depositor
    Jason Aoto
    Insert
    GFP-Cre recombinase
    Use
    AAV
    Tags
    Cre is fused to GFP. GFP is fused to additional n…
    Expression
    Mutation
    Promoter
    human synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175381-plasmid-map-sequence-id-348392

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  16. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid
    #200661
    Purpose
    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina
    Depositor
    Samer Hattar
    Insert
    DREADD Gq-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    200661-plasmid-map-sequence-id-393501

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  20. AAV-DIO-TVA-2A-oG

    Plasmid
    #178431
    Purpose
    Expresses TVA and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950
    Optimized G
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178431-plasmid-map-sequence-id-354735

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  21. pAAV-hSyn-con/fon DREADD Gi-mCherry

    Plasmid
    #200660
    Purpose
    Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina
    Depositor
    Samer Hattar
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    200660-plasmid-map-sequence-id-393500

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  24. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid
    #185699
    Purpose
    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter
    Depositor
    Lynette Lim
    Inserts
    Tdtomato,
    Synaptophysin-EGFP
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    mDlx
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185699-plasmid-map-sequence-id-366333

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  25. pLP-mOlig2-ires-gfp

    Plasmid
    #49399
    Purpose
    mammalian bicistronic expression of mouse Olig2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Olig2 (Olig2 Mouse)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP and IRES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    49399-plasmid-map-sequence-id-316898

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  26. AAV-DIO-TC66T-2A-oG

    Plasmid
    #178430
    Purpose
    Expresses TVA(TC66T) and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178430-plasmid-map-sequence-id-354734

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  27. pAAV-FLEX-C-RG

    Plasmid
    #49101
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under EF1a promoter, in AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV and Cre/Lox
    Tags
    cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    49101-plasmid-map-sequence-id-91148

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  29. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  39. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid
    #185966
    Purpose
    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185966-plasmid-map-sequence-id-367346

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  40. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid
    #185965
    Purpose
    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185965-plasmid-map-sequence-id-367345

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  41. pB6-Rosa26-cre_dre_reporter_1_1a

    Plasmid
    #185964
    Purpose
    B6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185964-plasmid-map-sequence-id-367344

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  42. pB6-Rosa26-cre_dre_reporter_1_1

    Plasmid
    #185963
    Purpose
    B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    EGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185963-plasmid-map-sequence-id-367343

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  43. pBSIIKS_HIS3_I

    Plasmid
    #109062
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109062-plasmid-map-sequence-id-209541

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  44. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

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  50. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

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Showing: 901 - 950 of 2919 results