We narrowed to 54,262 results for: Ran

1. pKDR-EF1a.2

   Plasmid

   #183569

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p3A9cbetaTCR.HSV2.3

   Plasmid

   #69594

   Purpose

   TCR beta chain genomic transgenic construct for generation of gBT-I mice

   Depositor

   Francis R. Carbone

   Article

   Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

   Insert

   TCR beta VJ region from clone HSV2.3 (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pENTR221-Renilla

   Plasmid

   #79496

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Renilla Luciferase
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

   Plasmid

   #194934

   Purpose

   LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. psiCHECK-2-Zfp36-3'UTR

   Plasmid

   #61780

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Zfp36

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Zfp36 3'UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLOT-syb-sp-YFP1-10

   Plasmid

   #73700

   Purpose

   Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP

   Depositor

   Marco Gallio

   Article

   Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

   Insert

   Neuronal-synaptobrevin:spYFP1–10 chimera
   

   Use

   Lexaop reporter vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a - Mtb-TF

   Plasmid

   #111513

   Purpose

   Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   Trigger Factor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR221-sGFP

    Plasmid

    #79508

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    superGFP
    

    Use

    Multisite gateway entry clone

    Tags

    6xHis

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac K-M33

    Plasmid

    #1986

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

12. p1DT (chicken beta 1 integrin)

    Plasmid

    #14066

    Depositor

    Richard Hynes

    Article

    Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

    Insert

    Chicken Beta 1 Integrin (ITGB1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pBTK300

    Plasmid

    #110592

    Purpose

    BTK Type 4 terminator for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    rpoC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pColE1_sgRNA_M7NS_AmpR

    Plasmid

    #119157

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pColE1_sgRNA_M2S_AmpR

    Plasmid

    #119154

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTC-CMV-Tet

    Plasmid

    #85577

    Purpose

    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liver

    Depositor

    Ursula Ehmer

    Article

    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

    Insert

    CreER
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ApoE.HCR.hAAT

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-flag-UTR

    Plasmid

    #183234

    Purpose

    Mammalian expression empty backbone

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a - CPX

    Plasmid

    #89620

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Cytosolic Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-rhodopsin-CTS

    Plasmid

    #86071

    Purpose

    A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Rhodopsin (RHO Bovine)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 309-348 of Rhodopsin

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPO640

    Plasmid

    #195296

    Purpose

    pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    miniMcCAST (Tn7575) with KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBDR

    Plasmid

    #62663

    Purpose

    Donor vector for tdTomato knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AY10_pS.Donor.R5.TS

    Plasmid

    #100292

    Purpose

    CCR5-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLdSaCN

    Plasmid

    #123261

    Purpose

    Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

    Insert

    gRNA and SaCas9
    

    Use

    CRISPR; Leishmania

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBTK206

    Plasmid

    #110588

    Purpose

    BTK Type 3 coding sequence for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Nanoluc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBMN-flag beta-catenin K19R/K49R

    Plasmid

    #44751

    Depositor

    Eric Fearon

    Article

    Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

    Insert

    beta-catenin
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K19R/K49R

    Promoter

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET21a - SodB

    Plasmid

    #89613

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase B
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hisSUMO-mZFP57

    Plasmid

    #87224

    Purpose

    Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    ZFP57 (Zfp57 Mouse)
    

    Use

    Tags

    hisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-GATA2-DNzf

    Plasmid

    #1291

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S263G and M434V

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

29. Clover-MyosinIIA-C-18

    Plasmid

    #56372

    Purpose

    Localization: Cytoskeleton, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    MyosinIIA
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. SB53

    Plasmid

    #21488

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1-65 deleted

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only