Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

174,131 results

Showing: 5701 - 5730 of 174131 results

  2. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

    Enlarge

  3. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

    Enlarge

  5. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

    Enlarge

  6. xlox(GFP)hTERT

    Plasmid
    #69809
    Purpose
    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a GFP marker
    Depositor
    David Ott
    Insert
    hTERT Reverse Transcriptase (TERT )
    Use
    Retroviral
    Tags
    none
    Promoter
    MLV promoter
    Available Since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    69809-plasmid-map-sequence-id-127377

    Enlarge

  7. pMito-rxRFP1.1

    Plasmid
    #67841
    Purpose
    redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized
    Depositor
    Huiwang Ai
    Article
    Fan et al Anal Bioanal Chem. 2016 Jan 12.
    Insert
    rxRFP1.1 and mitochondrial localization sequence
    Tags
    His Tag and mitochondrial localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    67841-plasmid-map-sequence-id-244286

    Enlarge

  9. OPRM1-Tango

    Plasmid
    #66464
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    FLAG and tTA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66464-plasmid-map-sequence-id-157903

    Enlarge

  11. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

    Enlarge

  17. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55005-plasmid-map-sequence-id-456544

    Enlarge

  21. mEmerald-Alpha-V-Integrin-25

    Plasmid
    #53985
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Alpha-V Integrin (ITGAV Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53985-plasmid-map-sequence-id-257384

    Enlarge

  22. GABA(A) receptor subunit a1SE

    Plasmid
    #49168
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    Tags
    myc and pHGFP (pHIuorin)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49168-plasmid-map-sequence-id-72484

    Enlarge

  25. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46826-plasmid-map-sequence-id-246440

    Enlarge

Showing: 5701 - 5730 of 174131 results